[Histonet] Pre-floating tissue sections in dilute alcohol

[Joana Moreira]

I Garrey,
I came across floating sections in dilute alcohol back in 2005 when I started working in the UK and have been using it ever since.
I agree it introduces one more step and wouldn't use for every single section - there's no need. But I do find it very helpful with certain blocks/tissues - especially the very 'wrinkly' ones.

When I started cutting during my training, we used other technique to avoid wrinkles (and waste of tissue sections) and produce 'perfect' sections. We used to have a small cold water bath to float and pick up the sections before transferring to the temperature controlled water bath.

Joana

Joana Moreira
Supervisor - Anatomical Pathology
Department of Pathology

Sidra Medical & Research Center
PO Box 26999 | Doha, Qatar
Direct Line  +974-4404-2036
jmoreira at sidra.org | www.sidra.org



Message: 12
Date: Sat, 6 Jun 2015 10:29:03 -0400
From: Garrey Faller <garreyf at gmail.com>
To: histonet at lists.utsouthwestern.edu
Subject: [Histonet] Pre-floating tissue sections in dilute alcohol
Message-ID: <A7285EF9-FA0F-4121-AA4C-78DFA5F1CE4E at gmail.com>
Content-Type: text/plain;       charset=us-ascii

Hi everyone,

I just became aware of this technique last week, and it seems to work great.
I did a quick google search and found this quick reference.
http://www.scielo.cl/pdf/ijmorphol/v30n1/art07.pdf <http://www.scielo.cl/pdf/ijmorphol/v30n1/art07.pdf>
Anyone out there float their sections in dilute ethyl alcohol before transferring to the water bath?
Its an extra step and introduces an extra chance to introduce floaters.
But, the quality seems to be improved.
Any thoughts?

Thanks in advance.
Garrey Faller
Pathologist

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