[Histonet] Pre-floating tissue sections in dilute alcohol

[tjfinney2010@gmail.com]

Yes I use it for brain.  

Happy Connecting.  Sent from my Sprint Phone.


------ Original message------
From: Garrey Faller
Date: Sat, Jun 6, 2015 10:48 AM
To: histonet at lists.utsouthwestern.edu;
Subject:[Histonet] Pre-floating tissue sections in dilute alcohol

Hi everyone,

I just became aware of this technique last week, and it seems to work great.
I did a quick google search and found this quick reference.
http://www.scielo.cl/pdf/ijmorphol/v30n1/art07.pdf 
Anyone out there float their sections in dilute ethyl alcohol before transferring to the water bath?
Its an extra step and introduces an extra chance to introduce floaters.
But, the quality seems to be improved.
Any thoughts?  

Thanks in advance.
Garrey Faller
Pathologist
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