[Histonet] Breast Fixation

[Garreyf]

I agree.  That's the easiest solution.

My colleague at a sister institution does not allow breast excisions on Friday.

We need more people to do studies on fixation beyond 72 hours. My hunch is that another 24 hours won't affect ER , Pgr, and Her2 results.

Garrey

Sent from my iPhone

> On Jul 29, 2015, at 5:01 PM, Joanne Clark via Histonet <histonet at lists.utsouthwestern.edu> wrote:
> 
> 
> I would do a delayed start on your tissue processor Friday night to include the extra two hours you need of fixation time and just have the run come off two hours later on Saturday morning.  Just adjust the hours of your per diem Saturday tech to come in later.
> 
> Joanne Clark, HT
> Director of Histology
> Pathology Consultants of New Mexico
> 
> 
> Message: 4
> Date: Wed, 29 Jul 2015 08:01:31 -0700
> From: "Heckford, Karen - SMMC-SF" <Karen.Heckford at DignityHealth.org>
> To: "histonet at lists.utsouthwestern.edu"
>    <histonet at lists.utsouthwestern.edu>
> Subject: [Histonet] Breast fixation
> Message-ID:
>    <D329219D3B967447A9E3ED3242117E3206FEDC7378 at PHX-MSG-007-N1.chw.edu>
> Content-Type: text/plain; charset="us-ascii"
> 
> Good Morning,
> I have a question about breast fixation.   I am in a little bit of a pickle with the 6-72 hour rule for the fixation on breast tissue.   Friday I am getting 2 breast cases in the afternoon and both will not have the required minimum 6 hour formalin fixation for my per diem to cut early Saturday morning.   He will not be able to make it in again until Monday night.  The tissue will be about 3-4 hours (this includes time on the processor)  over the 72 hour maximum.    Does anyone have any suggestions on what can be done?  We are a one person show here.
> 
> Thanks,
> 
> Karen Heckford HT ASCP CE
> Lead Histology Technician
> St. Mary's Medical Center
> 450 Stanyan St.
> San Francisco, Ca. 94117
> 415-668-1000 ext. 6167
> karen.heckford at dignityhealth.org
>                                                                                  Caution:  This email message, including all content and attachments, is CONFIDENTIAL and may be of a nature that is LEGALLY PRIVILEGED.  The information contained in this email message is intended only for the use of the recipient(s) named above. If the reader of this message is not the intended recipient or an agent responsible for delivering it to the intended recipient, you have received this document in error.  Any further review, dissemination, distribution, or copying of this message is strictly prohibited.  If you have received this communication in error, please notify us  immediately by reply email.  Thank you."
> 
> 
> 
> 
> 
> ------------------------------
> 
> Message: 5
> Date: Wed, 29 Jul 2015 12:10:19 -0300
> From: Emily Dewar <emilydewar32144 at gmail.com>
> To: histonet at lists.utsouthwestern.edu
> Subject: [Histonet] NetWell inserts and IHC with TUNEL stain
> Message-ID:
>    <CAJ7MPPp=h9pgEkH-SxJG=KkDiDxi4=6_TTrAP7RCb5NSJnm47A at mail.gmail.com>
> Content-Type: text/plain; charset=UTF-8
> 
> Hello,
> 
> I will be performing a number of immunos within the next couple of months,
> and have been wondering what the best way to do so might be with no tissue
> damage during the process. Does anyone know whether using NetWell inserts
> for immunos to transfer tissue affects morphology? The tissue will be
> placed into the insert, and submersed in solution within a well plate to be
> incubated on a shaker. The problem is that contact with the NetWell insert
> could damage the tissue, not only with the rocking, but with transfer from
> one solution to another.
> 
> I am under the impression that with TUNEL staining, is often difficult to
> differentiate whether the damage in cells caused by handling/extraction, or
> from the treatment itself. While not impossible, I will not have the time
> to develop or perform such a procedure.
> 
> If anyone has any knowledge or insight, it would be greatly appreciated.
> 
> Thank you for your time,
> 
> Emily Dewar
> Laboratory Technician
> 
> 
> ------------------------------
> 
> Subject: Digest Footer
> 
> _______________________________________________
> Histonet mailing list
> Histonet at lists.utsouthwestern.edu
> http://cp.mcafee.com/d/k-Kr6h8idEIcL8CzB55AsrKrhKyYO-ev7fCQrELcLzD4QjqdQnCnNPUVMSztZxN5xCVEVdydzZyl1iREaYKrmJ1nBPqavTd791Z_HYCyCO-esWZOWqbz7cccT7cfsJt6OaaJQSel3PWApmU6CS3r1K_9Tso73xParNKVI06vaAWsTeBmVAP7QDYotW3u2qKMM-l9OwXn2sGjG3jpel9R1FsxlK5LE2zVkDjCVQGTcCo-A_z3LgrdLcee6MLaAWwQQg1QyfQC0pEw3djPh0yNs0Ph06QETVEwphdII6N00eEd9Y
> 
> ------------------------------
> 
> End of Histonet Digest, Vol 140, Issue 31
> *****************************************
> 
> 
> 
> Disclaimer: This electronic message may contain information that is proprietary,
> confidential, or legally privileged or protected. It is intended only for the use
> of the individual(s) and entity named in the message. If you are not an intended
> recipient of this message, please notify the sender immediately and delete the
> material from your computer. Do not deliver, distribute or copy this message and
> do not disclose its contents or take any action in reliance on the information it
> contains.
> 
> _______________________________________________
> Histonet mailing list
> Histonet at lists.utsouthwestern.edu
> http://lists.utsouthwestern.edu/mailman/listinfo/histonet