[Histonet] Breast Fixation

Joanne Clark jclark at pcnm.com
Wed Jul 29 16:01:27 CDT 2015

I would do a delayed start on your tissue processor Friday night to include the extra two hours you need of fixation time and just have the run come off two hours later on Saturday morning.  Just adjust the hours of your per diem Saturday tech to come in later.

Joanne Clark, HT
Director of Histology
Pathology Consultants of New Mexico

Message: 4
Date: Wed, 29 Jul 2015 08:01:31 -0700
From: "Heckford, Karen - SMMC-SF" <Karen.Heckford at DignityHealth.org>
To: "histonet at lists.utsouthwestern.edu"
	<histonet at lists.utsouthwestern.edu>
Subject: [Histonet] Breast fixation
	<D329219D3B967447A9E3ED3242117E3206FEDC7378 at PHX-MSG-007-N1.chw.edu>
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Good Morning,
I have a question about breast fixation.   I am in a little bit of a pickle with the 6-72 hour rule for the fixation on breast tissue.   Friday I am getting 2 breast cases in the afternoon and both will not have the required minimum 6 hour formalin fixation for my per diem to cut early Saturday morning.   He will not be able to make it in again until Monday night.  The tissue will be about 3-4 hours (this includes time on the processor)  over the 72 hour maximum.    Does anyone have any suggestions on what can be done?  We are a one person show here.


Karen Heckford HT ASCP CE
Lead Histology Technician
St. Mary's Medical Center
450 Stanyan St.
San Francisco, Ca. 94117
415-668-1000 ext. 6167
karen.heckford at dignityhealth.org
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Message: 5
Date: Wed, 29 Jul 2015 12:10:19 -0300
From: Emily Dewar <emilydewar32144 at gmail.com>
To: histonet at lists.utsouthwestern.edu
Subject: [Histonet] NetWell inserts and IHC with TUNEL stain
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I will be performing a number of immunos within the next couple of months,
and have been wondering what the best way to do so might be with no tissue
damage during the process. Does anyone know whether using NetWell inserts
for immunos to transfer tissue affects morphology? The tissue will be
placed into the insert, and submersed in solution within a well plate to be
incubated on a shaker. The problem is that contact with the NetWell insert
could damage the tissue, not only with the rocking, but with transfer from
one solution to another.

I am under the impression that with TUNEL staining, is often difficult to
differentiate whether the damage in cells caused by handling/extraction, or
from the treatment itself. While not impossible, I will not have the time
to develop or perform such a procedure.

If anyone has any knowledge or insight, it would be greatly appreciated.

Thank you for your time,

Emily Dewar
Laboratory Technician


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