[Histonet] Masson's on Frozens.
rsrichmond at gmail.com
Fri Jul 17 19:01:54 CDT 2015
Bernice Frederick HTL (ASCP) at Northwestern in Chicago USA asks:
>>When we stain and H&E on frozen [sections] we fix in 95% prior to
staining. As I never in 30 years have done a Masson's Trichrome on a
frozen section I ask: do I need to fix and then mordant as usual or go
straight to the Bouin's and continue as normal? Do I need to mordant at
all? Tissue is rabbit liver.<<
Are you communicating with your pathologist? What are you trying to do?
The one-step trichrome techniques associated with the hallowed name of
George Gomori (particularly the Engel-Cunningham variant) are probably
preferable for frozen sections - they certainly are for skeletal muscle -
where I have a lot of experience, though many years ago. But find out
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