[Histonet] Masson's on Frozens.

Fri Jul 17 07:53:28 CDT 2015

Question:
When we stain and H&E on frozen we fix in 95% prior to staining. As I never in 30 years have done a Masson's Trichrome on a  frozen section I ask : do I need to fix and then mordant as usual or go straight to the  Bouin's and continue as normal? Do I need to mordant at all? Tissue is rabbit liver.
BErnice

Bernice Frederick HTL (ASCP)
Senior Research Tech
Pathology Core Facility
Robert. H. Lurie Cancer Center
Northwestern University
710 N Fairbanks Court
Olson 8-421
Chicago,IL 60611
312-503-3723
b-frederick at northwestern.edu<mailto:b-frederick at northwestern.edu>