[Histonet] Amyloid by Congo Red

[Jeffrey Robinson]

Greetings to all Histotechs-  Here's an amyloid question for the braintrust.  We are cutting our slides and controls at 9 and staining in Congo Red for 1 hour.  The control stains fine but the patient tissue is staining negative even on cases that the pathologist assures us should be positive for amyloid.  We are using the Leica APEX charged slides with control and patient tissue on the same slide.  Does anyone have any thoughts on why the patient tissue is not staining?  Thanks!

Jeff Robinson HT, HTL, Senior Histotechnologist, Sierra Pathology Lab, Clovis, CA.


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