[Histonet] RE: embedding

[Victoria Baker]

I have found really hot molds more difficult to work with but it is really
an individual preference.
What has worked for me is - put just enough paraffin in base mold to come
up to rim of embedding area.  Embed tissue.  Add just a little more
paraffin to bring it up to where plastic cassette top comes into contact
with hot paraffin. Tilt mold gently to spread paraffin.  Press cassette top
down and add enough paraffin to cassette top to come up half way. Do 5 - 6
blocks and then top off with hot paraffin.
The paraffin has had a chance to harden just enough to where it won't run
out from under the cassette and remain pretty clean.
Everyone has there tricks you just need to see what works best for you.
Vikki
 On Jan 21, 2015 10:28 AM, "Anne Murvosh" <amurvosh <@t> advancederm.net> wrote:

> My first job the molds were kept room temp or cool and I didn't have to
> scrape as much. My 2nd job they were kept hot and had to scrape all the
> time.  Have fun with it and test some cold, some room temp, some hot see if
> there is a difference. The problem with too cool molds is it takes a bit
> longer to embed cause the bottom takes longer to warm up so it's a trade
> off for time. Fun experiment though!  Anne
>
> -----Original Message-----
> From: histonet-bounces <@t> lists.utsouthwestern.edu [mailto:
> histonet-bounces <@t> lists.utsouthwestern.edu] On Behalf Of Campbell, Tasha M.
> Sent: Wednesday, January 21, 2015 7:12 AM
> To: histonet <@t> lists.utsouthwestern.edu
> Subject: [Histonet] embedding
>
> Hello everyone, I was just curious if anyone had tips on how to embed
> without getting paraffin on the outside of the cassettes so I don't have to
> scrape the blocks or at least not scrape very much.  Thanks!!
>
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> Tasha Campbell, B.S.,HTL(ASCP)
>
> Frederick Gastroenterology Associates
>
> 310 W. 9th St.
>
> Frederick, MD 21701
>
> 301-695-6800 ext. 144 (w)
>
> 304-685-9307 (c)
>
>
>
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