[Histonet] Heme "boogers"

[Ingles Claire]

Hello All!



We have been having an interesting problem with our Hematoxylin. It seems there are globules that form on the bottom of our hematoxylin wells in our automatic stainer (Leica XL). These globs are acellular, so it is not a fungus or something. We use a progressive Mayer's Hematoxylin formula as follows:



1000ml DI

50g Aluminum Ammonium Sulfate

1g Hematoxylin powder

1g Citric Acid

0.2g Sodium Iodate



Anyone know what this might be? It doesn't affect the staining, but likes to hang on the bottom of the staining racks and is a bit gross. Plus it gets dragged into some of the following wells on occasion.



Thanks!

Claire