[Histonet] Heme "boogers"

[Hans B Snyder]

That's the aluminum ammonium precipitating out, it happens with Harris also.  Do you filter it before use?  


Histologistics
Hans B Snyder
508.308.7800
Hans <@t> histologistics.com

> On Jan 15, 2015, at 16:46, Ingles Claire <CIngles <@t> uwhealth.org> wrote:
> 
> Hello All!
> 
> 
> 
> We have been having an interesting problem with our Hematoxylin. It seems there are globules that form on the bottom of our hematoxylin wells in our automatic stainer (Leica XL). These globs are acellular, so it is not a fungus or something. We use a progressive Mayer's Hematoxylin formula as follows:
> 
> 
> 
> 1000ml DI
> 
> 50g Aluminum Ammonium Sulfate
> 
> 1g Hematoxylin powder
> 
> 1g Citric Acid
> 
> 0.2g Sodium Iodate
> 
> 
> 
> Anyone know what this might be? It doesn't affect the staining, but likes to hang on the bottom of the staining racks and is a bit gross. Plus it gets dragged into some of the following wells on occasion.
> 
> 
> 
> Thanks!
> 
> Claire
> 
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