[Histonet] And other crazy stuff.

[Paula Sicurello]

I forgot to mention:   dirt

Happy Friday

Paula

On Fri, Jan 9, 2015 at 2:39 PM, Shirley A. Powell <POWELL_SA <@t> mercer.edu>
wrote:

> Orangutan testicle macro section and alligator jawbones, not my best work,
> very humbling, after 52 years in the business.
> Shirley
>
>
> -----Original Message-----
> From: histonet-bounces <@t> lists.utsouthwestern.edu [mailto:
> histonet-bounces <@t> lists.utsouthwestern.edu] On Behalf Of Morken, Timothy
> Sent: Tuesday, January 06, 2015 2:24 PM
> To: Patsy Ruegg; Roberta Horner; Douglas Gregg; Histonet <@t> Lists. Edu
> Subject: And other crazy stuff. RE: [Histonet] cutting honey bees
>
> You crazy research people...OK, so what is the craziest thing you ever had
> to cut, or were asked to cut?
>
> For me, not too bad, but embedding for EM and sectioning a single oocyte
> that was nearly microscopic. I'll just say it took a LOT of thick sections
> too face down to it without actually cutting through it.
>
>
> Open the floodgates....
>
> Tim Morken
>
> -----Original Message-----
> From: histonet-bounces <@t> lists.utsouthwestern.edu [mailto:
> histonet-bounces <@t> lists.utsouthwestern.edu] On Behalf Of Patsy Ruegg
> Sent: Tuesday, January 06, 2015 11:13 AM
> To: Roberta Horner; Douglas Gregg; Histonet <@t> Lists. Edu
> Subject: RE: [Histonet] cutting honey bees
>
> for the whole bee I probably would process and embed it in glycol
> methacrylate (gma) it is much harder and would give better sections, we
> have done zebra fish and several other harder tissues including calcified
> bone in GMA.
>
> Cheers,
> Patsy
>
> Patsy Ruegg, HT(ASCP)QIHC
> Ruegg IHC Consulting
> 40864 E Arkansas Ave
> Bennett, CO 80102
> H 303-644-4538
> C 720-281-5406
> pruegghm <@t> hotmail.com
>
>
>
> > From: rjr6 <@t> psu.edu
> > To: classicdoc <@t> gmail.com; histonet <@t> lists.utsouthwestern.edu
> > Date: Sat, 3 Jan 2015 23:15:33 +0000
> > Subject: RE: [Histonet] cutting honey bees
> > CC:
> >
> > I sectioned and stained honey bee and yellow jacket stingers years ago.
> They wanted to show the difference between the stingers.  I wasn't sure
> what to do so I processed and handled like everything else.  I was able to
> get some good sections.  I put 6 stingers in each block and cut several
> sections figuring there should be at least one good stinger in each block
> and it worked.
> > Roberta Horner
> > Penn State University
> > Animal Diagnostic Lab
> > ________________________________________
> > From: histonet-bounces <@t> lists.utsouthwestern.edu
> > [histonet-bounces <@t> lists.utsouthwestern.edu] on behalf of Douglas Gregg
> > [classicdoc <@t> gmail.com]
> > Sent: Saturday, January 03, 2015 6:08 PM
> > To: histonet <@t> lists.utsouthwestern.edu
> > Subject: [Histonet] cutting honey bees
> >
> > Has anyone had experience embedding and cutting honey bees. I am sure
> > there are some issues with the harder exoskeleton. Would that have to
> > be dissected away first. I am considering helping a student with a
> > science fair project on bees.
> >
> > Douglas Gregg
> > Veterianary pathologist
> >
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