[Histonet] And other crazy stuff.

[Hans B Snyder]

 You crazy research people...OK, so what is the craziest thing you
ever had to cut, or were asked to cut?

For me, I work in a research facility where I do everything.  One day
a botanist emailed me to cut pine on the cryostat and asked what the
largest size should be.  I told him 2 mm x 2mm and explained the
process as he had no idea what I was going to do. I thought ok, what
is pine?  He brought 5 pieces of wood pieces (pine) and asked if they
could be cut cross section at 10um with 5 sections per slide.  Of
course this is not something I have ever had expience in doing, and
they are hard as a rock.  The trick that ended up working, was to soak
the wood in warm water for 30 minutes, embed in OCT and cut.  After
cutting the sections were all balled up, so I floated them on water
and picked them up on the slide.  The sections were still folded and
looked horiffic in my mind but he loved them.  He said was going to
look at them under a Raman confocal and wanted to see the plant cell
wall.
Hans B Snyder
Histologistics
60 Prescott Street
Worcester, MA 01605
508-308-7800
hans <@t> histologistics.com


On Fri, Jan 9, 2015 at 2:39 PM, Shirley A. Powell <POWELL_SA <@t> mercer.edu> wrote:
> Orangutan testicle macro section and alligator jawbones, not my best work, very humbling, after 52 years in the business.
> Shirley
>
>
> -----Original Message-----
> From: histonet-bounces <@t> lists.utsouthwestern.edu [mailto:histonet-bounces <@t> lists.utsouthwestern.edu] On Behalf Of Morken, Timothy
> Sent: Tuesday, January 06, 2015 2:24 PM
> To: Patsy Ruegg; Roberta Horner; Douglas Gregg; Histonet <@t> Lists. Edu
> Subject: And other crazy stuff. RE: [Histonet] cutting honey bees
>
> You crazy research people...OK, so what is the craziest thing you ever had to cut, or were asked to cut?
>
> For me, not too bad, but embedding for EM and sectioning a single oocyte that was nearly microscopic. I'll just say it took a LOT of thick sections too face down to it without actually cutting through it.
>
>
> Open the floodgates....
>
> Tim Morken
>
> -----Original Message-----
> From: histonet-bounces <@t> lists.utsouthwestern.edu [mailto:histonet-bounces <@t> lists.utsouthwestern.edu] On Behalf Of Patsy Ruegg
> Sent: Tuesday, January 06, 2015 11:13 AM
> To: Roberta Horner; Douglas Gregg; Histonet <@t> Lists. Edu
> Subject: RE: [Histonet] cutting honey bees
>
> for the whole bee I probably would process and embed it in glycol methacrylate (gma) it is much harder and would give better sections, we have done zebra fish and several other harder tissues including calcified bone in GMA.
>
> Cheers,
> Patsy
>
> Patsy Ruegg, HT(ASCP)QIHC
> Ruegg IHC Consulting
> 40864 E Arkansas Ave
> Bennett, CO 80102
> H 303-644-4538
> C 720-281-5406
> pruegghm <@t> hotmail.com
>
>
>
>> From: rjr6 <@t> psu.edu
>> To: classicdoc <@t> gmail.com; histonet <@t> lists.utsouthwestern.edu
>> Date: Sat, 3 Jan 2015 23:15:33 +0000
>> Subject: RE: [Histonet] cutting honey bees
>> CC:
>>
>> I sectioned and stained honey bee and yellow jacket stingers years ago.  They wanted to show the difference between the stingers.  I wasn't sure what to do so I processed and handled like everything else.  I was able to get some good sections.  I put 6 stingers in each block and cut several sections figuring there should be at least one good stinger in each block and it worked.
>> Roberta Horner
>> Penn State University
>> Animal Diagnostic Lab
>> ________________________________________
>> From: histonet-bounces <@t> lists.utsouthwestern.edu
>> [histonet-bounces <@t> lists.utsouthwestern.edu] on behalf of Douglas Gregg
>> [classicdoc <@t> gmail.com]
>> Sent: Saturday, January 03, 2015 6:08 PM
>> To: histonet <@t> lists.utsouthwestern.edu
>> Subject: [Histonet] cutting honey bees
>>
>> Has anyone had experience embedding and cutting honey bees. I am sure
>> there are some issues with the harder exoskeleton. Would that have to
>> be dissected away first. I am considering helping a student with a
>> science fair project on bees.
>>
>> Douglas Gregg
>> Veterianary pathologist
>>
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