And other crazy stuff. RE: [Histonet] cutting honey bees

Thu Jan 8 14:41:55 CST 2015

we once played an april's fool joke on our Heme Pathologist.  We took the wings off a fly and embedded it in plastic (GMA) sectioned and stained it. Grossly It looked similar to the bone marrow core biopsies we did.  Heme pathologist are notorious for sticking slides under the microscope on high power without looking at them grossly.  We said we needed him to consult on this really strange bone marrow core because we couldn't figure out what the patient had.  Most of our patients at the time were patients with leukemia or lymphoma.  Sure enough he stuck the slide under high power and probably even under oil immersion.  Trying to look at the morphology of individual cells.  He was stumped and was going to show it to another colleague, so we had to confess that it was a fly and this was a AF joke.  We thought he would have thought we were clever and laugh about it but he did not think it was funny.  We never did anything like that again.

Patsy Ruegg, HT(ASCP)QIHC
Ruegg IHC Consulting
40864 E Arkansas Ave
Bennett, CO 80102
H 303-644-4538
C 720-281-5406
pruegghm <@t> hotmail.com

> Subject: RE: And other crazy stuff.  RE: [Histonet] cutting honey bees
> Date: Thu, 8 Jan 2015 15:27:58 -0500
> From: dhewitt <@t> hvhs.org
> To: mjones <@t> metropath.com; Timothy.Morken <@t> ucsf.edu; pruegghm <@t> hotmail.com; rjr6 <@t> psu.edu; classicdoc <@t> gmail.com; histonet <@t> lists.utsouthwestern.edu
> 
> I have done a stink bug, spider and a few other creepy crawlers for my
> kids to look at under the scope, they have no idea what they are looking
> at but still love it.
> 
> Daniel Hewitt
> Histology Supervisor, HVS
> 412-749-7371
> 
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> 
> -----Original Message-----
> From: histonet-bounces <@t> lists.utsouthwestern.edu
> [mailto:histonet-bounces <@t> lists.utsouthwestern.edu] On Behalf Of Michael
> Ann Jones
> Sent: Thursday, January 08, 2015 3:20 PM
> To: Morken, Timothy; Patsy Ruegg; Roberta Horner; Douglas Gregg;
> 'histonet <@t> lists.utsouthwestern.edu'
> Subject: Re: And other crazy stuff. RE: [Histonet] cutting honey bees
> 
> We did a goldfish once, interesting microscopically and difficult for
> peeling (lots of keratin?)
> Michael Ann Jones, HT (ASCP)
> Histology Manager
> Metropath
> 7444 W. Alaska Dr. #250
> Lakewood, CO 80226
> 303.634.2511
> Mjones <@t> metropath.com
> 
> 
> 
> 
> On 1/6/15, 12:23 PM, "Morken, Timothy" <Timothy.Morken <@t> ucsf.edu> wrote:
> 
> >You crazy research people...OK, so what is the craziest thing you ever
> >had to cut, or were asked to cut?
> >
> >For me, not too bad, but embedding for EM and sectioning a single
> oocyte
> >that was nearly microscopic. I'll just say it took a LOT of thick
> >sections too face down to it without actually cutting through it.
> >
> >
> >Open the floodgates....
> >
> >Tim Morken
> >
> >-----Original Message-----
> >From: histonet-bounces <@t> lists.utsouthwestern.edu
> >[mailto:histonet-bounces <@t> lists.utsouthwestern.edu] On Behalf Of Patsy
> >Ruegg
> >Sent: Tuesday, January 06, 2015 11:13 AM
> >To: Roberta Horner; Douglas Gregg; Histonet <@t> Lists. Edu
> >Subject: RE: [Histonet] cutting honey bees
> >
> >for the whole bee I probably would process and embed it in glycol
> >methacrylate (gma) it is much harder and would give better sections, we
> >have done zebra fish and several other harder tissues including
> calcified
> >bone in GMA.
> >
> >Cheers,
> >Patsy
> >
> >Patsy Ruegg, HT(ASCP)QIHC
> >Ruegg IHC Consulting
> >40864 E Arkansas Ave
> >Bennett, CO 80102
> >H 303-644-4538
> >C 720-281-5406
> >pruegghm <@t> hotmail.com
> >
> >
> >
> >> From: rjr6 <@t> psu.edu
> >> To: classicdoc <@t> gmail.com; histonet <@t> lists.utsouthwestern.edu
> >> Date: Sat, 3 Jan 2015 23:15:33 +0000
> >> Subject: RE: [Histonet] cutting honey bees
> >> CC: 
> >> 
> >> I sectioned and stained honey bee and yellow jacket stingers years
> ago.
> >> They wanted to show the difference between the stingers.  I wasn't
> sure
> >>what to do so I processed and handled like everything else.  I was
> able
> >>to get some good sections.  I put 6 stingers in each block and cut
> >>several sections figuring there should be at least one good stinger in
> >>each block and it worked.
> >> Roberta Horner
> >> Penn State University
> >> Animal Diagnostic Lab
> >> ________________________________________
> >> From: histonet-bounces <@t> lists.utsouthwestern.edu
> >> [histonet-bounces <@t> lists.utsouthwestern.edu] on behalf of Douglas
> Gregg
> >> [classicdoc <@t> gmail.com]
> >> Sent: Saturday, January 03, 2015 6:08 PM
> >> To: histonet <@t> lists.utsouthwestern.edu
> >> Subject: [Histonet] cutting honey bees
> >> 
> >> Has anyone had experience embedding and cutting honey bees. I am sure
> >> there are some issues with the harder exoskeleton. Would that have to
> >> be dissected away first. I am considering helping a student with a
> >> science fair project on bees.
> >> 
> >> Douglas Gregg
> >> Veterianary pathologist
> >> 
> >> _______________________________________________
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> >> Histonet <@t> lists.utsouthwestern.edu
> >> http://lists.utsouthwestern.edu/mailman/listinfo/histonet
> >> 
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