And other crazy stuff. RE: [Histonet] cutting honey bees

[Daniel Hewitt]

I have done a stink bug, spider and a few other creepy crawlers for my
kids to look at under the scope, they have no idea what they are looking
at but still love it.

Daniel Hewitt
Histology Supervisor, HVS
412-749-7371

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-----Original Message-----
From: histonet-bounces <@t> lists.utsouthwestern.edu
[mailto:histonet-bounces <@t> lists.utsouthwestern.edu] On Behalf Of Michael
Ann Jones
Sent: Thursday, January 08, 2015 3:20 PM
To: Morken, Timothy; Patsy Ruegg; Roberta Horner; Douglas Gregg;
'histonet <@t> lists.utsouthwestern.edu'
Subject: Re: And other crazy stuff. RE: [Histonet] cutting honey bees

We did a goldfish once, interesting microscopically and difficult for
peeling (lots of keratin?)
Michael Ann Jones, HT (ASCP)
Histology Manager
Metropath
7444 W. Alaska Dr. #250
Lakewood, CO 80226
303.634.2511
Mjones <@t> metropath.com




On 1/6/15, 12:23 PM, "Morken, Timothy" <Timothy.Morken <@t> ucsf.edu> wrote:

>You crazy research people...OK, so what is the craziest thing you ever
>had to cut, or were asked to cut?
>
>For me, not too bad, but embedding for EM and sectioning a single
oocyte
>that was nearly microscopic. I'll just say it took a LOT of thick
>sections too face down to it without actually cutting through it.
>
>
>Open the floodgates....
>
>Tim Morken
>
>-----Original Message-----
>From: histonet-bounces <@t> lists.utsouthwestern.edu
>[mailto:histonet-bounces <@t> lists.utsouthwestern.edu] On Behalf Of Patsy
>Ruegg
>Sent: Tuesday, January 06, 2015 11:13 AM
>To: Roberta Horner; Douglas Gregg; Histonet <@t> Lists. Edu
>Subject: RE: [Histonet] cutting honey bees
>
>for the whole bee I probably would process and embed it in glycol
>methacrylate (gma) it is much harder and would give better sections, we
>have done zebra fish and several other harder tissues including
calcified
>bone in GMA.
>
>Cheers,
>Patsy
>
>Patsy Ruegg, HT(ASCP)QIHC
>Ruegg IHC Consulting
>40864 E Arkansas Ave
>Bennett, CO 80102
>H 303-644-4538
>C 720-281-5406
>pruegghm <@t> hotmail.com
>
>
>
>> From: rjr6 <@t> psu.edu
>> To: classicdoc <@t> gmail.com; histonet <@t> lists.utsouthwestern.edu
>> Date: Sat, 3 Jan 2015 23:15:33 +0000
>> Subject: RE: [Histonet] cutting honey bees
>> CC: 
>> 
>> I sectioned and stained honey bee and yellow jacket stingers years
ago.
>> They wanted to show the difference between the stingers.  I wasn't
sure
>>what to do so I processed and handled like everything else.  I was
able
>>to get some good sections.  I put 6 stingers in each block and cut
>>several sections figuring there should be at least one good stinger in
>>each block and it worked.
>> Roberta Horner
>> Penn State University
>> Animal Diagnostic Lab
>> ________________________________________
>> From: histonet-bounces <@t> lists.utsouthwestern.edu
>> [histonet-bounces <@t> lists.utsouthwestern.edu] on behalf of Douglas
Gregg
>> [classicdoc <@t> gmail.com]
>> Sent: Saturday, January 03, 2015 6:08 PM
>> To: histonet <@t> lists.utsouthwestern.edu
>> Subject: [Histonet] cutting honey bees
>> 
>> Has anyone had experience embedding and cutting honey bees. I am sure
>> there are some issues with the harder exoskeleton. Would that have to
>> be dissected away first. I am considering helping a student with a
>> science fair project on bees.
>> 
>> Douglas Gregg
>> Veterianary pathologist
>> 
>> _______________________________________________
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