And other crazy stuff. RE: [Histonet] cutting honey bees

[Paula Sicurello]

You asked for it:

squid tentacle tip, frog oocyte, serial sections through a entire zebra
fish embryo, honey bee eyes, harbor seal artery, Andean mummy muscle (flaky
little boogers), probably lots of others that I can't remember off the top
of my head.

Paula

On Thu, Jan 8, 2015 at 3:20 PM, Michael Ann Jones <mjones <@t> metropath.com>
wrote:

> We did a goldfish once, interesting microscopically and difficult for
> peeling (lots of keratin?)
> Michael Ann Jones, HT (ASCP)
> Histology Manager
> Metropath
> 7444 W. Alaska Dr. #250
> Lakewood, CO 80226
> 303.634.2511
> Mjones <@t> metropath.com
>
>
>
>
> On 1/6/15, 12:23 PM, "Morken, Timothy" <Timothy.Morken <@t> ucsf.edu> wrote:
>
> >You crazy research people...OK, so what is the craziest thing you ever
> >had to cut, or were asked to cut?
> >
> >For me, not too bad, but embedding for EM and sectioning a single oocyte
> >that was nearly microscopic. I'll just say it took a LOT of thick
> >sections too face down to it without actually cutting through it.
> >
> >
> >Open the floodgates....
> >
> >Tim Morken
> >
> >-----Original Message-----
> >From: histonet-bounces <@t> lists.utsouthwestern.edu
> >[mailto:histonet-bounces <@t> lists.utsouthwestern.edu] On Behalf Of Patsy
> >Ruegg
> >Sent: Tuesday, January 06, 2015 11:13 AM
> >To: Roberta Horner; Douglas Gregg; Histonet <@t> Lists. Edu
> >Subject: RE: [Histonet] cutting honey bees
> >
> >for the whole bee I probably would process and embed it in glycol
> >methacrylate (gma) it is much harder and would give better sections, we
> >have done zebra fish and several other harder tissues including calcified
> >bone in GMA.
> >
> >Cheers,
> >Patsy
> >
> >Patsy Ruegg, HT(ASCP)QIHC
> >Ruegg IHC Consulting
> >40864 E Arkansas Ave
> >Bennett, CO 80102
> >H 303-644-4538
> >C 720-281-5406
> >pruegghm <@t> hotmail.com
> >
> >
> >
> >> From: rjr6 <@t> psu.edu
> >> To: classicdoc <@t> gmail.com; histonet <@t> lists.utsouthwestern.edu
> >> Date: Sat, 3 Jan 2015 23:15:33 +0000
> >> Subject: RE: [Histonet] cutting honey bees
> >> CC:
> >>
> >> I sectioned and stained honey bee and yellow jacket stingers years ago.
> >> They wanted to show the difference between the stingers.  I wasn't sure
> >>what to do so I processed and handled like everything else.  I was able
> >>to get some good sections.  I put 6 stingers in each block and cut
> >>several sections figuring there should be at least one good stinger in
> >>each block and it worked.
> >> Roberta Horner
> >> Penn State University
> >> Animal Diagnostic Lab
> >> ________________________________________
> >> From: histonet-bounces <@t> lists.utsouthwestern.edu
> >> [histonet-bounces <@t> lists.utsouthwestern.edu] on behalf of Douglas Gregg
> >> [classicdoc <@t> gmail.com]
> >> Sent: Saturday, January 03, 2015 6:08 PM
> >> To: histonet <@t> lists.utsouthwestern.edu
> >> Subject: [Histonet] cutting honey bees
> >>
> >> Has anyone had experience embedding and cutting honey bees. I am sure
> >> there are some issues with the harder exoskeleton. Would that have to
> >> be dissected away first. I am considering helping a student with a
> >> science fair project on bees.
> >>
> >> Douglas Gregg
> >> Veterianary pathologist
> >>
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