And other crazy stuff. RE: [Histonet] cutting honey bees

[Michael Ann Jones]

We did a goldfish once, interesting microscopically and difficult for
peeling (lots of keratin?)
Michael Ann Jones, HT (ASCP)
Histology Manager
Metropath
7444 W. Alaska Dr. #250
Lakewood, CO 80226
303.634.2511
Mjones <@t> metropath.com




On 1/6/15, 12:23 PM, "Morken, Timothy" <Timothy.Morken <@t> ucsf.edu> wrote:

>You crazy research people...OK, so what is the craziest thing you ever
>had to cut, or were asked to cut?
>
>For me, not too bad, but embedding for EM and sectioning a single oocyte
>that was nearly microscopic. I'll just say it took a LOT of thick
>sections too face down to it without actually cutting through it.
>
>
>Open the floodgates....
>
>Tim Morken
>
>-----Original Message-----
>From: histonet-bounces <@t> lists.utsouthwestern.edu
>[mailto:histonet-bounces <@t> lists.utsouthwestern.edu] On Behalf Of Patsy
>Ruegg
>Sent: Tuesday, January 06, 2015 11:13 AM
>To: Roberta Horner; Douglas Gregg; Histonet <@t> Lists. Edu
>Subject: RE: [Histonet] cutting honey bees
>
>for the whole bee I probably would process and embed it in glycol
>methacrylate (gma) it is much harder and would give better sections, we
>have done zebra fish and several other harder tissues including calcified
>bone in GMA.
>
>Cheers,
>Patsy
>
>Patsy Ruegg, HT(ASCP)QIHC
>Ruegg IHC Consulting
>40864 E Arkansas Ave
>Bennett, CO 80102
>H 303-644-4538
>C 720-281-5406
>pruegghm <@t> hotmail.com
>
>
>
>> From: rjr6 <@t> psu.edu
>> To: classicdoc <@t> gmail.com; histonet <@t> lists.utsouthwestern.edu
>> Date: Sat, 3 Jan 2015 23:15:33 +0000
>> Subject: RE: [Histonet] cutting honey bees
>> CC: 
>> 
>> I sectioned and stained honey bee and yellow jacket stingers years ago.
>> They wanted to show the difference between the stingers.  I wasn't sure
>>what to do so I processed and handled like everything else.  I was able
>>to get some good sections.  I put 6 stingers in each block and cut
>>several sections figuring there should be at least one good stinger in
>>each block and it worked.
>> Roberta Horner
>> Penn State University
>> Animal Diagnostic Lab
>> ________________________________________
>> From: histonet-bounces <@t> lists.utsouthwestern.edu
>> [histonet-bounces <@t> lists.utsouthwestern.edu] on behalf of Douglas Gregg
>> [classicdoc <@t> gmail.com]
>> Sent: Saturday, January 03, 2015 6:08 PM
>> To: histonet <@t> lists.utsouthwestern.edu
>> Subject: [Histonet] cutting honey bees
>> 
>> Has anyone had experience embedding and cutting honey bees. I am sure
>> there are some issues with the harder exoskeleton. Would that have to
>> be dissected away first. I am considering helping a student with a
>> science fair project on bees.
>> 
>> Douglas Gregg
>> Veterianary pathologist
>> 
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