[Histonet] CD68 and CD204 for dogs/pigs

[Mehmet Fatih BOZKURT]

Hello Jan,  I will stain ED1 (cd68) for you on dog tissue Friday. I hope it
works.

On Wed, Feb 25, 2015 at 7:42 PM, Jan Shivers <shive003 <@t> umn.edu> wrote:

> Hi all,
>
> I do have MAC387 and it works great in dog and pig tissue.  The PI is
> asking for additional CD markers to macrophages (CD68 and 204).  I need
> them to work on dogs and pigs, but of course, there's not many vendors who
> list species cross-reactivities on their data sheets beyond the usual human
> and mouse.
>
> In my earlier trials years ago with CD68 (clone KP1), I didn't have success
> using it on dog tissue.  Am looking for a different clone that will
> cross-react with dogs.
>
> Jan
>
> On Wed, Feb 25, 2015 at 11:08 AM, Elizabeth Chlipala <liz <@t> premierlab.com>
> wrote:
>
> > Jan
> >
> > MAC387 is a marker that we have used successfully in canine and porcine,
> > not entirely specific to macs but may work for your situation.
> >
> > LIz
> >
> > Elizabeth A. Chlipala, BS, HTL(ASCP)QIHC
> > Premier Laboratory, LLC
> > PO Box 18592
> > Boulder, CO 80308
> > (303) 682-3949 office
> > (303) 682-9060 fax
> > (303) 881-0763 cell
> > liz <@t> premierlab.com
> > www.premierlab.com
> >
> > March 10, 2014 is Histotechnology Professionals Day
> >
> > Ship to Address:
> >
> > Premier Laboratory, LLC
> > 1567 Skyway Drive, Unit E
> > Longmont, CO 80504
> >
> > -----Original Message-----
> > From: histonet-bounces <@t> lists.utsouthwestern.edu [mailto:
> > histonet-bounces <@t> lists.utsouthwestern.edu] On Behalf Of Jan Shivers
> > Sent: Tuesday, February 24, 2015 3:59 PM
> > To: histonet
> > Subject: [Histonet] CD68 and CD204 for dogs/pigs
> >
> > Can anyone recommend vendors for CD68 and CD204 that work on dogs and
> pigs
> > (on FFPE tissue)?  I don't seem to find much information on species
> > cross-reactivity online.
> >
> > Thanks much in advance.
> >
> > --
> > Jan Shivers
> > Senior Scientist
> > IHC/Histology Section Head
> > Pathology Teaching Program
> > Veterinary Diagnostic Laboratory
> > University of Minnesota
> > 1333 Gortner Ave.
> > St. Paul, MN  55108
> > 612-624-7297
> > shive003 <@t> umn.edu
> > _______________________________________________
> > Histonet mailing list
> > Histonet <@t> lists.utsouthwestern.edu
> > http://lists.utsouthwestern.edu/mailman/listinfo/histonet
> >
>
>
>
> --
> Jan Shivers
> Senior Scientist
> IHC/Histology Section Head
> Pathology Teaching Program
> Veterinary Diagnostic Laboratory
> University of Minnesota
> 1333 Gortner Ave.
> St. Paul, MN  55108
> 612-624-7297
> shive003 <@t> umn.edu
> _______________________________________________
> Histonet mailing list
> Histonet <@t> lists.utsouthwestern.edu
> http://lists.utsouthwestern.edu/mailman/listinfo/histonet
>



-- 
Asst. Prof. Dr. Mehmet Fatih BOZKURT
Department of Pathology
Faculty of Veterinary Medicine
Afyon Kocatepe University
03100, ANS Campus
Afyonkarahisar-TURKEY
Tel: +902722281312-16173/16237