[Histonet] CD68 and CD204 for dogs/pigs

[Julio Benavides]

Hi,

MAC387 is a great marker but it detects less macrophages than CD68. In 
our experience (sheep and cattle), MAC387 is good for labelling 
macrophages that have recently reach the lesion, while those resident or 
long standing macrophages would be negative to MAC387 (but mostly 
positive to CD68, which is a more general marker for macs). No idea 
about CD204 though.

Just in case this helps

Regards

Julio

On 25/02/2015 18:08, Elizabeth Chlipala wrote:
> Jan
>
> MAC387 is a marker that we have used successfully in canine and porcine, not entirely specific to macs but may work for your situation.
>
> LIz
>
> Elizabeth A. Chlipala, BS, HTL(ASCP)QIHC
> Premier Laboratory, LLC
> PO Box 18592
> Boulder, CO 80308
> (303) 682-3949 office
> (303) 682-9060 fax
> (303) 881-0763 cell
> liz <@t> premierlab.com
> www.premierlab.com
>
> March 10, 2014 is Histotechnology Professionals Day
>
> Ship to Address:
>
> Premier Laboratory, LLC
> 1567 Skyway Drive, Unit E
> Longmont, CO 80504
>
> -----Original Message-----
> From: histonet-bounces <@t> lists.utsouthwestern.edu [mailto:histonet-bounces <@t> lists.utsouthwestern.edu] On Behalf Of Jan Shivers
> Sent: Tuesday, February 24, 2015 3:59 PM
> To: histonet
> Subject: [Histonet] CD68 and CD204 for dogs/pigs
>
> Can anyone recommend vendors for CD68 and CD204 that work on dogs and pigs (on FFPE tissue)?  I don't seem to find much information on species cross-reactivity online.
>
> Thanks much in advance.
>
> --
> Jan Shivers
> Senior Scientist
> IHC/Histology Section Head
> Pathology Teaching Program
> Veterinary Diagnostic Laboratory
> University of Minnesota
> 1333 Gortner Ave.
> St. Paul, MN  55108
> 612-624-7297
> shive003 <@t> umn.edu
> _______________________________________________
> Histonet mailing list
> Histonet <@t> lists.utsouthwestern.edu
> http://lists.utsouthwestern.edu/mailman/listinfo/histonet
>
>
> _______________________________________________
> Histonet mailing list
> Histonet <@t> lists.utsouthwestern.edu
> http://lists.utsouthwestern.edu/mailman/listinfo/histonet