[Histonet] CD68 and CD204 for dogs/pigs

[Jan Shivers]

Hi all,

I do have MAC387 and it works great in dog and pig tissue.  The PI is
asking for additional CD markers to macrophages (CD68 and 204).  I need
them to work on dogs and pigs, but of course, there's not many vendors who
list species cross-reactivities on their data sheets beyond the usual human
and mouse.

In my earlier trials years ago with CD68 (clone KP1), I didn't have success
using it on dog tissue.  Am looking for a different clone that will
cross-react with dogs.

Jan

On Wed, Feb 25, 2015 at 11:08 AM, Elizabeth Chlipala <liz <@t> premierlab.com>
wrote:

> Jan
>
> MAC387 is a marker that we have used successfully in canine and porcine,
> not entirely specific to macs but may work for your situation.
>
> LIz
>
> Elizabeth A. Chlipala, BS, HTL(ASCP)QIHC
> Premier Laboratory, LLC
> PO Box 18592
> Boulder, CO 80308
> (303) 682-3949 office
> (303) 682-9060 fax
> (303) 881-0763 cell
> liz <@t> premierlab.com
> www.premierlab.com
>
> March 10, 2014 is Histotechnology Professionals Day
>
> Ship to Address:
>
> Premier Laboratory, LLC
> 1567 Skyway Drive, Unit E
> Longmont, CO 80504
>
> -----Original Message-----
> From: histonet-bounces <@t> lists.utsouthwestern.edu [mailto:
> histonet-bounces <@t> lists.utsouthwestern.edu] On Behalf Of Jan Shivers
> Sent: Tuesday, February 24, 2015 3:59 PM
> To: histonet
> Subject: [Histonet] CD68 and CD204 for dogs/pigs
>
> Can anyone recommend vendors for CD68 and CD204 that work on dogs and pigs
> (on FFPE tissue)?  I don't seem to find much information on species
> cross-reactivity online.
>
> Thanks much in advance.
>
> --
> Jan Shivers
> Senior Scientist
> IHC/Histology Section Head
> Pathology Teaching Program
> Veterinary Diagnostic Laboratory
> University of Minnesota
> 1333 Gortner Ave.
> St. Paul, MN  55108
> 612-624-7297
> shive003 <@t> umn.edu
> _______________________________________________
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>



-- 
Jan Shivers
Senior Scientist
IHC/Histology Section Head
Pathology Teaching Program
Veterinary Diagnostic Laboratory
University of Minnesota
1333 Gortner Ave.
St. Paul, MN  55108
612-624-7297
shive003 <@t> umn.edu