[Histonet] RE: sections not sticking to charged slides

[Debra Siena]

Hi Renee,

Hope you don't mind but I have had a lot of experience in this area.  I worked for Ventana for 7 1/2 years as technical specialist and now as a Technical Support Manager and  I know that getting sections to stick to slides can be a bit challenging especially during antigen retrieval.  One of the rules when using positively charged slides is to not use any type of adhesive in the water bath, such as Sta-On only use distilled water.  The use of such products actually works against you when trying to get the sections adhered to slides.  Also I would say that when doing IHC, something to keep in mind is that adhesive properties of the slides vary over time, so age of the slides is a factor and also exposure to humidity and heat is another factor which can affect the adhesive properties of the slides.  Make sure that you are not buying too many slides at any one time and that you go through them in no more than 6 months.  Also if cutting controls and storing in a plastic box that can mean that the slides sit around for awhile before being used, so don't cut too many controls at one time.  I would suggest drying the slides for at least 30 minutes to 1 hour at 60C and if you are getting trapped water under the section, you may need to dry longer to make sure that all the water is removed.  

If you would like to sample some of the StatLab slides that we feel are very good for IHC, please let myself or Kevin Collins know, we would be happy to sample them if you haven't already tried them.  If I can help in any other way, please let me know.  



  Debbie Siena, HT(ASCP)QIHC
StatLab Medical Products
Technical Support Manager
407 Interchange Street | McKinney, TX 75071
t: 800.442.3573 ext. 229 | f: 972.767.3992
dsiena <@t> statlab.com | www.statlab.com

-----Original Message-----
From: histonet-bounces <@t> lists.utsouthwestern.edu [mailto:histonet-bounces <@t> lists.utsouthwestern.edu] On Behalf Of Renee H. Workman
Sent: Tuesday, February 24, 2015 3:33 PM
To: histonet <@t> lists.utsouthwestern.edu
Subject: [Histonet] sections not sticking to charged slides


Help, sections not sticking to charged slides.  We use Mercedes Medical charged slides.  Lately have been using sta-on but still have occasional problems especially during antigen retrieval.  I need any suggestions.

Renee H. Workman
Histology Supervisor
Virginia Urology
9105 Stony Point Drive
Richmond, VA  23235
W: 804-527-1316 | F: 804-270-0917
rhworkman <@t> uro.com | www.uro.com





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