[Histonet] RE: sections not sticking to charged slides

[Jeffrey Robinson]

HI Renee-  I had a major problem in the past with tissue staining for IHC lifting off the slides no matter what I tried.  It turned out that when we precut our controls and put them in the oven and then used them later for the patient tissue that the charge on the slide was altered.  We currently still precut controls on control slides (we use Leica APEX) and then let them just air dry with no heat.  We then use those slides when needed and add the patient tissue to the bottom and then put the slides in the oven (we use 70C for one hour) prior to IHC staining.  The tissue lifting decreased dramatically- even on breast tissue.  Be sure to pick up your controls from the wrong (label) end when picking up your controls and do not let the bottom half of the slide get into the waterbath so as to avoid "double-dipping" which can also cause tissue adherence problems.

Jeff Robinson, Senior Histotechnologist, Sierra Pathology Lab, Clovis, CA.

-----Original Message-----
From: histonet-bounces <@t> lists.utsouthwestern.edu [mailto:histonet-bounces <@t> lists.utsouthwestern.edu] On Behalf Of Renee H. Workman
Sent: Tuesday, February 24, 2015 1:33 PM
To: histonet <@t> lists.utsouthwestern.edu
Subject: [Histonet] sections not sticking to charged slides


Help, sections not sticking to charged slides.  We use Mercedes Medical charged slides.  Lately have been using sta-on but still have occasional problems especially during antigen retrieval.  I need any suggestions.

Renee H. Workman
Histology Supervisor
Virginia Urology
9105 Stony Point Drive
Richmond, VA  23235
W: 804-527-1316 | F: 804-270-0917
rhworkman <@t> uro.com | www.uro.com





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