[Histonet] gelatin

[Yak-Nam Wang]

Thank you for your e-mail.

Apologies for not explaining "treated tissue". We treat the tissue with
high intensity focused ultrasound. It can raise the temperature of tissue
to boiling in a localized area (millimeter areas). I could use a
biochemical assay for collagen and gelatin if we treat a large area, but
with single lesions I was hoping I could visualize this. In some treated
areas we are almost resulting in liquefaction of the tissue. I am
interested to see if we are turning the collagen to gelatin in these areas
and what part of the lesion this is happening.

Thank you for your thoughts
Yak-Nam

On Mon, Feb 23, 2015 at 9:47 PM, John Kiernan <jkiernan <@t> uwo.ca> wrote:

> You need to explain "treated tissue".
>
> Gelatin is collagen that has been boiled until the protein has lost all
> its fibrous nature and changed into a water-soluble protein. Gelatin is
> made permanently insoluble by adequate formaldehyde fixation. It is stained
> by anionic dyes (such as eosin in the H&E method), but it does not show as
> fibres when you look at the section or smear through a microscope.
>
> If this doesn't answer your question, please explain your problem and
> involve your boss in future email exchanges.
>
> *John Kiernan*
> London, Canada
> = = =
>
> On 23/02/15, *Yak-Nam Wang * <ynwang <@t> u.washington.edu> wrote:
>
> Hello,
>
> Does anyone know of a stain specific for gelatin? I would like to
> distinguish between firbous collagen and gelatin in treated tissue.
>
> thank you
>
> Yak-Nam
>
> University of Washington
> Seattle, WA
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