[Histonet] How dark is dark enough?

[Paula Sicurello]

Good Morning Netters,

While running immunofluorescence stains, how dark is dark enough?

I have worked in labs where we did them in full room light, almost complete
darkness (developing negative/film darkness), and somewhere in between.

I feel that dimmed lights are good enough.

What does the histology collective think?

Thanks in advance!

Paula  :-)