[EXTERNAL] Re: [Histonet] gelatin

[Roy, Ryan]

That's interesting, I didn't realize gelatin is the water soluable protein of collagen. No experience with staining gelatin, but have you considered MassonTrichrome. 

It is used to differentiate collagen from smooth muscle...


-----Original Message-----
From: histonet-bounces <@t> lists.utsouthwestern.edu [mailto:histonet-bounces <@t> lists.utsouthwestern.edu] On Behalf Of John Kiernan
Sent: Tuesday, February 24, 2015 12:48 AM
To: Yak-Nam Wang; histonet <@t> lists.utsouthwestern.edu
Subject: [EXTERNAL] Re: [Histonet] gelatin

You need to explain "treated tissue". 

Gelatin is collagen that has been boiled until the protein has lost all its fibrous nature and changed into a water-soluble protein. Gelatin is made permanently insoluble by adequate formaldehyde fixation. It is stained by anionic dyes (such as eosin in the H&E method), but it does not show as fibres when you look at the section or smear through a microscope. 

If this doesn't answer your question, please explain your problem and involve your boss in future email exchanges.

John Kiernan
London, Canada
= = =
On 23/02/15, Yak-Nam Wang  <ynwang <@t> u.washington.edu> wrote:
> Hello,
> 
> Does anyone know of a stain specific for gelatin? I would like to 
> distinguish between firbous collagen and gelatin in treated tissue.
> 
> thank you
> 
> Yak-Nam
> 
> University of Washington
> Seattle, WA
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