[Histonet] How dark is dark enough?

[Rene J Buesa]

As long as your microscope has a good Hg source light, it is not really necessary to darken the room so much. I used to have a very small room were the microscope was located but later on we moved the microscope to another area were we did not dim the light at all and the vision was OK.Everything depends on the circumstances and how comfortable you are with the image.There are no rules on this subject.René J.  

     On Tuesday, February 24, 2015 10:21 AM, Paula Sicurello <patpxs <@t> gmail.com> wrote:
   

 Good Morning Netters,

While running immunofluorescence stains, how dark is dark enough?

I have worked in labs where we did them in full room light, almost complete
darkness (developing negative/film darkness), and somewhere in between.

I feel that dimmed lights are good enough.

What does the histology collective think?

Thanks in advance!

Paula  :-)
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