[Histonet] gelatin

John Kiernan jkiernan <@t> uwo.ca
Mon Feb 23 23:47:50 CST 2015

You need to explain "treated tissue". 

Gelatin is collagen that has been boiled until the protein has lost all its fibrous nature and changed into a water-soluble protein. Gelatin is made permanently insoluble by adequate formaldehyde fixation. It is stained by anionic dyes (such as eosin in the H&E method), but it does not show as fibres when you look at the section or smear through a microscope. 

If this doesn't answer your question, please explain your problem and involve your boss in future email exchanges.

John Kiernan
London, Canada
= = =
On 23/02/15, Yak-Nam Wang  <ynwang <@t> u.washington.edu> wrote:
> Hello,
> Does anyone know of a stain specific for gelatin? I would like to
> distinguish between firbous collagen and gelatin in treated tissue.
> thank you
> Yak-Nam
> University of Washington
> Seattle, WA
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