[Histonet] Cryosectioning undecalcified bone

[Elizabeth Chlipala]

Orla

There is an article in the Journal of Histotechnology from a while ago from John Tarpley that addressed methods for this.  I have a pdf of it and I will send in another e-mail.

Liz

Elizabeth A. Chlipala, BS, HTL(ASCP)QIHC
Premier Laboratory, LLC
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(303) 881-0763 cell
liz <@t> premierlab.com
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-----Original Message-----
From: histonet-bounces <@t> lists.utsouthwestern.edu [mailto:histonet-bounces <@t> lists.utsouthwestern.edu] On Behalf Of Orla M Gallagher
Sent: Thursday, February 05, 2015 10:56 AM
To: histonet <@t> lists.utsouthwestern.edu
Subject: [Histonet] Cryosectioning undecalcified bone

Dear Histonetters,

We would like to develop cryosectioning for undecalcified mouse and rat bones. Previous attempts to use the CryoJane system from Instrumedics with an old Bright cryostat and solid tungsten carbide blade a few years ago didn't result in successful reproducible sections  - marrow without bone or bone without marrow on the slide, in spite of various freezing and prep.
protocols used by my colleague. I suspect the old cryostat and blade weren't helping either.

Have you any recommendations on the best cryostat to use to do this? We'd like to also use the cryostat for standard soft tissue sectioning. I've seen Leica mentioned in a few papers relating to CryoJane.

There appears also to be a tape transfer method from Kawamoto's Section-Lab Co. Ltd. (info <@t> section-lab.jp)

Does anyone use this method or know whether the consumables are still available for purchase, as the website seems dormant?

Thanks for any advice,
Orla

--
**************************
Ms. Orla Gallagher
Bone Analysis Laboratory
Mellanby Centre for Bone Research
Department of Human Metabolism
D Floor Medical School
University of Sheffield
Beech Hill Road
Sheffield
S10 2RX
UK

Website: http://mellanbycentre.dept.shef.ac.uk

Tel:         0044114-2713337 (office)
              0044114-2713174 (lab)
E-Mail:    o.m.gallagher <@t> sheffield.ac.uk


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