[Histonet] Cryosectioning undecalcified bone

[Orla M Gallagher]

Dear Histonetters,

We would like to develop cryosectioning for undecalcified mouse and rat
bones. Previous attempts to use the CryoJane system from Instrumedics with
an old Bright cryostat and solid tungsten carbide blade a few years ago
didn't result in successful reproducible sections  - marrow without bone or
bone without marrow on the slide, in spite of various freezing and prep.
protocols used by my colleague. I suspect the old cryostat and blade
weren't helping either.

Have you any recommendations on the best cryostat to use to do this? We'd
like to also use the cryostat for standard soft tissue sectioning. I've
seen Leica mentioned in a few papers relating to CryoJane.

There appears also to be a tape transfer method from Kawamoto's Section-Lab
Co. Ltd. (info <@t> section-lab.jp)

Does anyone use this method or know whether the consumables are still
available for purchase, as the website seems dormant?

Thanks for any advice,
Orla

-- 
**************************
Ms. Orla Gallagher
Bone Analysis Laboratory
Mellanby Centre for Bone Research
Department of Human Metabolism
D Floor Medical School
University of Sheffield
Beech Hill Road
Sheffield
S10 2RX
UK

Website: http://mellanbycentre.dept.shef.ac.uk

Tel:         0044114-2713337 (office)
              0044114-2713174 (lab)
E-Mail:    o.m.gallagher <@t> sheffield.ac.uk


*STOP*: Do you really need to print this e-mail?

*BE GREEN:* Keep it on the screen.

http://www.shef.ac.uk/faculty/medicine-dentistry-health/2.9274/emailwording

http://www.sheffield.ac.uk/humanmetabolism/greenimpact
<http://www.shef.ac.uk/faculty/medicine-dentistry-health/2.9274/emailwording>

http://www.sheffield.ac.uk/visitors/mapsandtravel