[Histonet] paraffin sectioning-dry tissue?

[Caroline Miller]

Yes, exactly what Mike and Geoff said. All mouse tissue, especially liver, can be really dry and needs a 'soak'. I have left them for an hour before now but don't leave it for longer than 4 hours though because it can start to swell and de-process!

You will still only get a few non-chattery sections so be gentle. Thinner sections also help too (3-4.5). Plus low um polishing after you trim

Good luck! It is weird at first but you will get used to it!

Caroline

Sent from my iPhone

> On Feb 5, 2015, at 6:29 AM, Geoff <mcauliff <@t> rwjms.rutgers.edu> wrote:
> 
> This is common with mouse and rat tissues, they get "over-dried" with a typical processing schedule.
> Soaking the face of the block with a kimwipe wet with ice water for 60 -120 seconds will enable you to cut 10 nice sections, maybe more.
> 
> Geoff
> 
>> On 2/5/2015 6:23 AM, Emily Brown wrote:
>> Hello all!
>> 
>> I just started sectioning mouse liver in paraffin and the tissue is very
>> dry.  I know it's not supposed to have water due to the processing, but the
>> weird thing is that one tech's solution is to put a wet kimwipe on the
>> block for a while.
>> It seems to me that there is a larger processing issue if this is
>> happening, am I correct? And why add water when you've already dehydrated
>> it?
>> Unfortunately, we do not have the set up to embed them ourselves, so we
>> have to send them to a histology lab.  They were sectioning for us, but
>> they are backlogged, so my boss wants me to do it.  Therefore, I can't tell
>> you how they were processed, but I think usually the histology lab manages
>> to get good sections.
>> Is putting a wet kimwipe (using distilled water) the best way to get rid of
>> chatter that's only in the tissue? The surrounding paraffin sections
>> excellent.
>> This may have been answered already, but a very quick google search didn't
>> help.  My googlefu is probably erratic as it's still early.
>> 
>> Emily
>> 
>> 
>> "By bitching and bitching and bitching, they could exhaust the drama of
>> their own horror stories. Grow bored. Only then could they accept a new
>> story for their lives. Move forward."
>> 
>> -Chuck Palahniuk, "Haunted"
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> 
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> Neuroscience and Cell Biology
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