[Histonet] paraffin sectioning-dry tissue?

Geoff mcauliff <@t> rwjms.rutgers.edu
Thu Feb 5 08:29:25 CST 2015

This is common with mouse and rat tissues, they get "over-dried" with a 
typical processing schedule.
Soaking the face of the block with a kimwipe wet with ice water for 60 
-120 seconds will enable you to cut 10 nice sections, maybe more.


On 2/5/2015 6:23 AM, Emily Brown wrote:
> Hello all!
> I just started sectioning mouse liver in paraffin and the tissue is very
> dry.  I know it's not supposed to have water due to the processing, but the
> weird thing is that one tech's solution is to put a wet kimwipe on the
> block for a while.
> It seems to me that there is a larger processing issue if this is
> happening, am I correct? And why add water when you've already dehydrated
> it?
> Unfortunately, we do not have the set up to embed them ourselves, so we
> have to send them to a histology lab.  They were sectioning for us, but
> they are backlogged, so my boss wants me to do it.  Therefore, I can't tell
> you how they were processed, but I think usually the histology lab manages
> to get good sections.
> Is putting a wet kimwipe (using distilled water) the best way to get rid of
> chatter that's only in the tissue? The surrounding paraffin sections
> excellent.
> This may have been answered already, but a very quick google search didn't
> help.  My googlefu is probably erratic as it's still early.
> Emily
> "By bitching and bitching and bitching, they could exhaust the drama of
> their own horror stories. Grow bored. Only then could they accept a new
> story for their lives. Move forward."
> -Chuck Palahniuk, "Haunted"
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Geoff McAuliffe, Ph.D.
Neuroscience and Cell Biology
Robert Wood Johnson Medical School
675 Hoes Lane, Piscataway, NJ 08854
voice: (732) 235-4583; fax: -4029
mcauliff <@t> rwjms.rutgers.edu

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