[Histonet] paraffin sectioning-dry tissue?

[Michael Ann Jones]

After macro trimming into our blocks (very gently if friable or dry) we
place them on an ice tray to soak for a good while.
Each well is filled with a little bit of water - this rehydrates the
tissue just enough to get a few good sections off the top.
we¹ve had success with this method for most any tissue (the more dry the
tissue, the longer the soak).

Michael Ann Jones, HT (ASCP)
Histology Manager
Metropath
7444 W. Alaska Dr. #250
Lakewood, CO 80226
303.634.2511
Mjones <@t> metropath.com







On 2/5/15, 4:23 AM, "Emily Brown" <talulahgosh <@t> gmail.com> wrote:

>Hello all!
>
>I just started sectioning mouse liver in paraffin and the tissue is very
>dry.  I know it's not supposed to have water due to the processing, but
>the
>weird thing is that one tech's solution is to put a wet kimwipe on the
>block for a while.
>It seems to me that there is a larger processing issue if this is
>happening, am I correct? And why add water when you've already dehydrated
>it?
>Unfortunately, we do not have the set up to embed them ourselves, so we
>have to send them to a histology lab.  They were sectioning for us, but
>they are backlogged, so my boss wants me to do it.  Therefore, I can't
>tell
>you how they were processed, but I think usually the histology lab manages
>to get good sections.
>Is putting a wet kimwipe (using distilled water) the best way to get rid
>of
>chatter that's only in the tissue? The surrounding paraffin sections
>excellent.
>This may have been answered already, but a very quick google search didn't
>help.  My googlefu is probably erratic as it's still early.
>
>Emily
>
>
>"By bitching and bitching and bitching, they could exhaust the drama of
>their own horror stories. Grow bored. Only then could they accept a new
>story for their lives. Move forward."
>
>-Chuck Palahniuk, "Haunted"
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