[Histonet] Over- decalcified tissue

[Roy, Lisa]

Good article on IHC world about restoring nuclear detail to over decalcified tissue....it is called Problem Number 23.  Good luck.

-----Original Message-----
From: histonet-bounces <@t> lists.utsouthwestern.edu [mailto:histonet-bounces <@t> lists.utsouthwestern.edu] On Behalf Of WILLIAM DESALVO
Sent: Wednesday, April 29, 2015 12:21 PM
To: pablo.sanchez <@t> usc.es
Cc: Histonet <@t> lists.utsouthwestern.edu
Subject: Re: [Histonet] Over- decalcified tissue

I would suggest not allowing decalcification to be extended. Better to leave in fixative until you can control the time of endpoint of decalcification. What type of decal solution are you using? I am not a big fan of trying to adjust the chemistry of the stain to compensate for over decalcification. Is there opportunity to submit another sample?

Sent from my iPhone

> On Apr 29, 2015, at 9:08 AM, pablo.sanchez <@t> usc.es wrote:
> 
> As I usually process gross pieces of bone -that need abouth thirty hours decalcifying- I suffer the same nuissance. Also would thank any hint.
> 
> Pablo Sanchez
> 
> 
> 
> Laurie Colbert <lcolbert <@t> pathmdlabs.com> escribiu:
> 
>> I have tissue that was left in decal over the weekend and now has very poor nuclear staining.  Is there a "fix" for this so that I can get better nuclear staining (other than restaining for a long time in the hematoxylin)?
>> 
>> Laurie Colbert, HT (ASCP)
>> Histology Supervisor
>> PATH MD
>> 8158 Beverly Blvd.
>> Los Angeles, CA  90048
>> (323) 648-3214 direct
>> (424) 245-7284 main lab
>> 
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