[Histonet] Over- decalcified tissue

[WILLIAM DESALVO]

I would suggest not allowing decalcification to be extended. Better to leave in fixative until you can control the time of endpoint of decalcification. What type of decal solution are you using? I am not a big fan of trying to adjust the chemistry of the stain to compensate for over decalcification. Is there opportunity to submit another sample?

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> On Apr 29, 2015, at 9:08 AM, pablo.sanchez <@t> usc.es wrote:
> 
> As I usually process gross pieces of bone -that need abouth thirty hours decalcifying- I suffer the same nuissance. Also would thank any hint.
> 
> Pablo Sanchez
> 
> 
> 
> Laurie Colbert <lcolbert <@t> pathmdlabs.com> escribiu:
> 
>> I have tissue that was left in decal over the weekend and now has very poor nuclear staining.  Is there a "fix" for this so that I can get better nuclear staining (other than restaining for a long time in the hematoxylin)?
>> 
>> Laurie Colbert, HT (ASCP)
>> Histology Supervisor
>> PATH MD
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>> Los Angeles, CA  90048
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