[Histonet] Over- decalcified tissue

[C.D.G.]

Laurie and Pablo, doing a soft antigen retrieval in a citrate buffer 
solution pH6 for 10 minutes after hydrating your slides
will allow you to improve the quality of nuclear stain.

El 29/04/2015 a las 13:07, pablo.sanchez <@t> usc.es escribió:
> As I usually process gross pieces of bone -that need abouth thirty 
> hours decalcifying- I suffer the same nuissance. Also would thank any 
> hint.
>
> Pablo Sanchez
>
>
>
> Laurie Colbert <lcolbert <@t> pathmdlabs.com> escribiu:
>
>> I have tissue that was left in decal over the weekend and now has 
>> very poor nuclear staining.  Is there a "fix" for this so that I can 
>> get better nuclear staining (other than restaining for a long time in 
>> the hematoxylin)?
>>
>> Laurie Colbert, HT (ASCP)
>> Histology Supervisor
>> PATH MD
>> 8158 Beverly Blvd.
>> Los Angeles, CA  90048
>> (323) 648-3214 direct
>> (424) 245-7284 main lab
>>
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