[Histonet] Over- decalcified tissue

[pablo.sanchez <@t> usc.es]

As I usually process gross pieces of bone -that need abouth thirty  
hours decalcifying- I suffer the same nuissance. Also would thank any  
hint.

Pablo Sanchez



Laurie Colbert <lcolbert <@t> pathmdlabs.com> escribiu:

> I have tissue that was left in decal over the weekend and now has  
> very poor nuclear staining.  Is there a "fix" for this so that I can  
> get better nuclear staining (other than restaining for a long time  
> in the hematoxylin)?
>
> Laurie Colbert, HT (ASCP)
> Histology Supervisor
> PATH MD
> 8158 Beverly Blvd.
> Los Angeles, CA  90048
> (323) 648-3214 direct
> (424) 245-7284 main lab
>
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