[Histonet] IHC and oven temperature

[WILLIAM DESALVO]

Dry heat compared to wet heat. Do not "dry" your slides at high heat. You are removing water trapped between slide and paraffin section. Antigen retrieval is an entirely different process. So not try to combine the two processes

Sent from my iPhone

> On Apr 20, 2015, at 8:48 AM, Preiszner, Johanna <PREISZNE <@t> mail.etsu.edu> wrote:
> 
> Hi Netters,
> 
> is there something wrong with this logic:
> 
> "If the tissue needs 95C for HIER, it's ok to dry the slides in an 82C oven."
> 
> Of course I'll test it before I try it on real specimens, but maybe someone else already knows the answer...
> 
> Thanks!
> 
> Hanna Preiszner
> ETSU/QCOM
> 
> 
> _______________________________________________
> Histonet mailing list
> Histonet <@t> lists.utsouthwestern.edu
> http://lists.utsouthwestern.edu/mailman/listinfo/histonet