[Histonet] IHC and oven temperature

[Tony Henwood (SCHN)]

Hi temp drying shown to be a bad idea:

Henwood, A., (2005) “Effect of Slide Drying at 80°C on Immunohistochemistry” J Histotechnol 28(1):45-46.

Abstract

Prolonged high temperature dry heating has been found to be deleterious to the immunohistochemical demonstration of several antigens in formalin-fixed, paraffin- embedded sections. Paraffin sections were dried at 80°C for 7 h and their immunoreactivity was compared with mirror sections dried for 1 h at 60°C. NCL-5D3, CMV, S100, HMB45, and CEA were quite labile to dry overheating whereas AElAE3, HBsAg, HBcAg, HSVII, EMA, chromogranin, and NSE were found to be quite resistant. It is recommended that coated slides (poly-L-lysine or aminopropyltriethoxysilane) and low-temperature drying (<60°C) be routinely used for irnmunohistochemistry.

________________________________________
From: histonet-bounces <@t> lists.utsouthwestern.edu [histonet-bounces <@t> lists.utsouthwestern.edu] on behalf of WILLIAM DESALVO [wdesalvo.cac <@t> outlook.com]
Sent: Tuesday, 21 April 2015 1:56 AM
To: Preiszner, Johanna
Cc: histonet <@t> lists.utsouthwestern.edu
Subject: Re: [Histonet] IHC and oven temperature

Dry heat compared to wet heat. Do not "dry" your slides at high heat. You are removing water trapped between slide and paraffin section. Antigen retrieval is an entirely different process. So not try to combine the two processes

Sent from my iPhone

> On Apr 20, 2015, at 8:48 AM, Preiszner, Johanna <PREISZNE <@t> mail.etsu.edu> wrote:
>
> Hi Netters,
>
> is there something wrong with this logic:
>
> "If the tissue needs 95C for HIER, it's ok to dry the slides in an 82C oven."
>
> Of course I'll test it before I try it on real specimens, but maybe someone else already knows the answer...
>
> Thanks!
>
> Hanna Preiszner
> ETSU/QCOM
>
>
> _______________________________________________
> Histonet mailing list
> Histonet <@t> lists.utsouthwestern.edu
> http://lists.utsouthwestern.edu/mailman/listinfo/histonet

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