[Histonet] RE: IHC and oven temperature

[Morken, Timothy]

The95 for HIER is in liquid, The 82in the oven is dry slides. While wet high temps enhance HIER, It seems the high dry temp does harm epitopes (there is a paper somewhere on this but I don't have access to it right now).



Tim Morken
Supervisor, Histology, Electron Microscopy and Neuromuscular Special Studies
UC San Francisco Medical Center
San Francisco, CA

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-----Original Message-----
From: histonet-bounces <@t> lists.utsouthwestern.edu [mailto:histonet-bounces <@t> lists.utsouthwestern.edu] On Behalf Of Preiszner, Johanna
Sent: Monday, April 20, 2015 8:47 AM
To: histonet <@t> lists.utsouthwestern.edu
Subject: [Histonet] IHC and oven temperature

Hi Netters,

is there something wrong with this logic:

"If the tissue needs 95C for HIER, it's ok to dry the slides in an 82C oven."

Of course I'll test it before I try it on real specimens, but maybe someone else already knows the answer...

Thanks!

Hanna Preiszner
ETSU/QCOM


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