[Histonet] RE: IHC and oven temperature

Morken, Timothy Timothy.Morken <@t> ucsf.edu
Mon Apr 20 10:52:53 CDT 2015


The95 for HIER is in liquid, The 82in the oven is dry slides. While wet high temps enhance HIER, It seems the high dry temp does harm epitopes (there is a paper somewhere on this but I don't have access to it right now).



Tim Morken
Supervisor, Histology, Electron Microscopy and Neuromuscular Special Studies
UC San Francisco Medical Center
San Francisco, CA

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-----Original Message-----
From: histonet-bounces <@t> lists.utsouthwestern.edu [mailto:histonet-bounces <@t> lists.utsouthwestern.edu] On Behalf Of Preiszner, Johanna
Sent: Monday, April 20, 2015 8:47 AM
To: histonet <@t> lists.utsouthwestern.edu
Subject: [Histonet] IHC and oven temperature

Hi Netters,

is there something wrong with this logic:

"If the tissue needs 95C for HIER, it's ok to dry the slides in an 82C oven."

Of course I'll test it before I try it on real specimens, but maybe someone else already knows the answer...

Thanks!

Hanna Preiszner
ETSU/QCOM


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