[Histonet] Maximum size of tissue for paraffin processing

[Rene J Buesa]

I am not aware of any such studies [dealing with specimen sizes] but it is a known fact since early XXth century that large specimens [such as complete brain slices] are known to be processed and yes, they require extended dehydrtaion → clearing → infiltration but not much more than usual IF the slices are thin enough, and yes, automated tissue processors can be used [I have done them for brain and whole prostates].René J.  


     On Thursday, April 9, 2015 2:26 PM, Caroline Miller <mills <@t> 3scan.com> wrote:
   

 Hi there wonderful Histonet people,

Has anyone seen any studies on the maximum size of tissue that can be
paraffin (or resin) processed. I am not talking about the size that can fit
in a tissue cassette, but, for example, entire pig bladder processing.

I realize it would include extended processing time, temp, vacuum,
agitation so imagine that none of these factors were limiting as I shall be
using an automated tissue processor.

I also realize that this is going to be tissue dependent. I was hoping
there were already some studies in this area that I am not finding in both
google and pubmed searches.

thanks, in advance, for any links you can point me to,

yours,
Caroline

-- 
Caroline Miller
Director of Histology
3Scan.com
415 2187297
_______________________________________________
Histonet mailing list
Histonet <@t> lists.utsouthwestern.edu
http://lists.utsouthwestern.edu/mailman/listinfo/histonet


   
     On Thursday, April 9, 2015 2:26 PM, Caroline Miller <mills <@t> 3scan.com> wrote:
   

 Hi there wonderful Histonet people,

Has anyone seen any studies on the maximum size of tissue that can be
paraffin (or resin) processed. I am not talking about the size that can fit
in a tissue cassette, but, for example, entire pig bladder processing.

I realize it would include extended processing time, temp, vacuum,
agitation so imagine that none of these factors were limiting as I shall be
using an automated tissue processor.

I also realize that this is going to be tissue dependent. I was hoping
there were already some studies in this area that I am not finding in both
google and pubmed searches.

thanks, in advance, for any links you can point me to,

yours,
Caroline

-- 
Caroline Miller
Director of Histology
3Scan.com
415 2187297
_______________________________________________
Histonet mailing list
Histonet <@t> lists.utsouthwestern.edu
http://lists.utsouthwestern.edu/mailman/listinfo/histonet