[Histonet] Maximum size of tissue for paraffin processing

[Elizabeth Chlipala]

We processed tissues as large as elk and lion vocal cords - 2 cm x 5 cm.  You just need to adjust the time accordingly.  Very large samples we would process manually through graded alcohols and then the last absolute, xylene and paraffin would be run on the tissue processor, we have programs that range from 6 to 24 hours a station depending upon tissue size.  You can run the entire process cycle on a tissue processor its just that we did not want to hang up one of the processors for a week on one study.

Liz

Elizabeth A. Chlipala, BS, HTL(ASCP)QIHC
Premier Laboratory, LLC
PO Box 18592
Boulder, CO 80308
(303) 682-3949 office
(303) 682-9060 fax
(303) 881-0763 cell
liz <@t> premierlab.com
www.premierlab.com

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-----Original Message-----
From: histonet-bounces <@t> lists.utsouthwestern.edu [mailto:histonet-bounces <@t> lists.utsouthwestern.edu] On Behalf Of Caroline Miller
Sent: Thursday, April 09, 2015 12:26 PM
To: Histonet <@t> Lists. Edu
Subject: [Histonet] Maximum size of tissue for paraffin processing

Hi there wonderful Histonet people,

Has anyone seen any studies on the maximum size of tissue that can be paraffin (or resin) processed. I am not talking about the size that can fit in a tissue cassette, but, for example, entire pig bladder processing.

I realize it would include extended processing time, temp, vacuum, agitation so imagine that none of these factors were limiting as I shall be using an automated tissue processor.

I also realize that this is going to be tissue dependent. I was hoping there were already some studies in this area that I am not finding in both google and pubmed searches.

thanks, in advance, for any links you can point me to,

yours,
Caroline

--
Caroline Miller
Director of Histology
3Scan.com
415 2187297
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