[Histonet] RE: Histonet Digest, Vol 136, Issue 13
Cynthia Hutchinson
tilycyn <@t> auburn.edu
Wed Apr 1 13:39:06 CDT 2015
I can have them ready tonight or tomorrow
Cynthia Tily Hutchinson
Rsch Asst IV
Pathobiology
166 Greene Hall
Coll of Vet Med
Auburn Univ 36849
(334)844 7020
-----Original Message-----
From: histonet-bounces <@t> lists.utsouthwestern.edu [mailto:histonet-bounces <@t> lists.utsouthwestern.edu] On Behalf Of histonet-request <@t> lists.utsouthwestern.edu
Sent: Wednesday, March 11, 2015 11:58 AM
To: histonet <@t> lists.utsouthwestern.edu
Subject: Histonet Digest, Vol 136, Issue 13
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Today's Topics:
1. anti-BRAF V600E (VE1) Ventana (Susan Foreman)
2. RE: RE: Old slides. (suetp918)
3. PRN (Kristy Castillo)
4. Re: anti-BRAF V600E (VE1) Ventana (Patrick Laurie)
5. Open histology positions: San Francisco (Cheryl)
6. Direct Hire Histotech South of Tucson, AZ-New Grads Welcome
to Apply! (Melissa Owens)
7. Job opportunity (Hunter, Theresa)
8. TDP43, PTG poly (victor_tobias <@t> comcast.net)
9. RE: Re: Bird head stored in 70% alcohol and possible
decalcification (Rui TAHARA)
10. BIG day today! (David Kemler)
11. PA (Mike)
12. Re: PA (Jennifer MacDonald)
13. Diff Quik troubleshooting (Nancy Schmitt)
14. THICK AND THIN SECTIONS ? (Klaus Dern)
15. RE: Old slides (Mayer,Toysha N)
16. 5-methylcytosine IHC in tissue (Mariela Chertoff)
17. RE: Masson Trichrome stain (Mayer,Toysha N)
18. RE: Old slides (Marcum, Pamela A)
19. RE: Histonet Digest, Vol 136, Issue 12 (Solis, Bryan)
----------------------------------------------------------------------
Message: 1
Date: Tue, 10 Mar 2015 13:15:05 -0400
From: "Susan Foreman" <sforeman <@t> labpath.com>
Subject: [Histonet] anti-BRAF V600E (VE1) Ventana
To: <histonet <@t> lists.utsouthwestern.edu>
Message-ID: <00bd01d05b55$c09b85f0$41d291d0$@labpath.com>
Content-Type: text/plain; charset="us-ascii"
What vendor are you guys using for antibody anti-BRAF V600E (VE1)? Spring
Bioscience or Ventana? What dilution are you using? Are you utilizing
amplification? Expensive
------------------------------
Message: 2
Date: Tue, 10 Mar 2015 13:31:58 -0400
From: suetp918 <suetp918 <@t> comcast.net>
Subject: RE: [Histonet] RE: Old slides.
To: "Gowan,Christie C" <christiecgowan <@t> dermatology.med.ufl.edu>,
'Bernice Frederick' <b-frederick <@t> northwestern.edu>,
"histonet <@t> lists.utsouthwestern.edu"
<histonet <@t> lists.utsouthwestern.edu>
Message-ID: <jqx50dw1pn5k224ex1acg53i.1426008646143 <@t> email.android.com>
Content-Type: text/plain; charset=utf-8
So we actually cut the film around the section and mount to another slide and do pretty much what wascabove mentioned placing upside downband placing on paper towel. ??Actually works pretty good.
TJUH
Sue Paturzo
Sent from my Verizon Wireless 4G LTE smartphone
-------- Original message --------
From: "Gowan,Christie C" <christiecgowan <@t> dermatology.med.ufl.edu>
Date:03/09/2015 4:01 PM (GMT-05:00)
To: 'Bernice Frederick' <b-frederick <@t> northwestern.edu>, histonet <@t> lists.utsouthwestern.edu
Subject: [Histonet] RE: Old slides.
Hi Bernice,
I have found that if you flood the slide with mounting media (don't use xylene) flip the slide over onto an absorbent lab wipe and put a heavy weight with even pressure and leave for a few hours. If the slide sticks to the wipe just put a few drops of xylene to clean up the slide. You may still have some tiny bubbles but it is much better than the alternative. Good luck.
Christie Gowan
-----Original Message-----
From: histonet-bounces <@t> lists.utsouthwestern.edu [mailto:histonet-bounces <@t> lists.utsouthwestern.edu] On Behalf Of Bernice Frederick
Sent: Monday, March 09, 2015 3:42 PM
To: histonet <@t> lists.utsouthwestern.edu
Subject: [Histonet] Old slides.
Hi all,
We received some old slides (1997-1998) that were coverslipped with film. Sakura I would imagine. The issue here is that the coverslips have come up from the slide and the tissue is adhered to the back of the coverslip. They need to be recovered so they can be evaluated. What do you all recommend? We use the CV5030 for coverslipping. I tried one with xylene and mounting media but there were still a couple of air bubbles in there.
Thanks,
Bernice
Bernice Frederick HTL (ASCP)
Senior Research Tech
Pathology Core Facility
Robert. H. Lurie Cancer Center
Northwestern University
710 N Fairbanks Court
Olson 8-421
Chicago,IL 60611
312-503-3723
b-frederick <@t> northwestern.edu<mailto:b-frederick <@t> northwestern.edu>
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------------------------------
Message: 3
Date: Tue, 10 Mar 2015 11:11:54 -0700
From: Kristy Castillo <kcastillo <@t> swskin.net>
Subject: [Histonet] PRN
To: "'histonet <@t> lists.utsouthwestern.edu'"
<histonet <@t> lists.utsouthwestern.edu>
Message-ID: <DED6D994175B70449148C3DEFCF034958C6119CB00 <@t> exchange>
Content-Type: text/plain; charset="us-ascii"
Hi Histonetters,
I am looking to fill a PRN Histotech position in the Phoenix area. Does anyone know anyone who is retired and looking for PRN Histology work? Please feel free to e-mail me at the following kcastillo <@t> swskin.net<mailto:kcastillo <@t> swskin.net> Thank you!
________________________________
This transmission may contain confidential information, some or all of which may be protected health information as defined by the federal Health Insurance Portability & Accountability Act (HIPAA) Privacy Rule. This transmission is intended for the exclusive use of the individual or entity to whom it is addressed and may contain information that is proprietary, privileged, confidential and/or exempt from disclosure under applicable law. If you are not the intended recipient (or an employee or agent responsible for delivering this transmission to the intended recipient), you are hereby notified that any disclosure, dissemination, distribution or copying of this information is strictly prohibited and may be subject to legal restriction or sanction. Please notify the sender by telephone to arrange the return or destruction of the information and all copies.
------------------------------
Message: 4
Date: Tue, 10 Mar 2015 14:48:23 -0400
From: Patrick Laurie <foreightl <@t> gmail.com>
Subject: Re: [Histonet] anti-BRAF V600E (VE1) Ventana
To: Susan Foreman <sforeman <@t> labpath.com>
Cc: "histonet <@t> lists.utsouthwestern.edu"
<histonet <@t> lists.utsouthwestern.edu>
Message-ID:
<CAKEyg-1hZDAZkjRByiqJaczx2gpn18TJGg_oQdi-0LYx+4edoA <@t> mail.gmail.com>
Content-Type: text/plain; charset=UTF-8
Spring Bioscience is part of Ventana, Spring had the original clone, and
now so does Ventana. We use the spring bioscience concentrated antibody on
the Bond III at 1:100 dilution, using the Bond Hi-pH ER2 retrieval for 20
minutes. I know that Ventana sells the RTU version. Remember that from
Spring it is RUO, so it is up to you to do the appropriate LDT. The
Ventana antibody is an IVD. It is also a pretty pricey antibody from either
vendor.
Let me know if you need any further help.
Patrick Laurie(HT)ASCP QIHC
Histology Manager
Celligent Diagnostics, LLC
101 East W.T. Harris Blvd | Suite 1212 | Charlotte, NC 28262
Work: 704-970-3300 Cell: 704-266-0869
On Tue, Mar 10, 2015 at 1:15 PM, Susan Foreman <sforeman <@t> labpath.com> wrote:
> What vendor are you guys using for antibody anti-BRAF V600E (VE1)? Spring
> Bioscience or Ventana? What dilution are you using? Are you utilizing
> amplification? Expensive
>
> _______________________________________________
> Histonet mailing list
> Histonet <@t> lists.utsouthwestern.edu
> http://lists.utsouthwestern.edu/mailman/listinfo/histonet
>
------------------------------
Message: 5
Date: Tue, 10 Mar 2015 12:37:23 -0700
From: Cheryl <tkngflght <@t> yahoo.com>
Subject: [Histonet] Open histology positions: San Francisco
To: histonet <@t> lists.utsouthwestern.edu
Message-ID:
<1426016243.35673.YahooMailBasic <@t> web161204.mail.bf1.yahoo.com>
Content-Type: text/plain; charset=us-ascii
Happy Histology Day, everyone!
I'm looking for techs for my new (to me) lab!! I'm working at CPMC Sutter in San Francisco and we need a couple of permanent hires for our team.
First few days it looks like it's gonna be fun - rock'n'roll kinda place where quality and speed have equal bearing.
Let me know what you're looking for and let's see if it's a match!
Email resumes to me at the email, below.
Please feel free to share and THANK YOU!
Cheryl Kerry, HT(ASCP)
admin <@t> fullstaff.org
------------------------------
Message: 6
Date: Tue, 10 Mar 2015 20:28:30 +0000
From: Melissa Owens <melissa <@t> alliedsearchpartners.com>
Subject: [Histonet] Direct Hire Histotech South of Tucson, AZ-New
Grads Welcome to Apply!
To: "histonet <@t> lists.utsouthwestern.edu"
<histonet <@t> lists.utsouthwestern.edu>
Message-ID: <D124CC2A.76634%melissa <@t> alliedsearchpartners.com>
Content-Type: text/plain; charset="us-ascii"
Hello Histonet,
I am in search of a histotech for a permanent position about an hour south of Tucson, AZ. New grads welcome to apply and relocation assistance is offered. Please contact me for more details. Thank you!
Melissa Owens
Allied Search Partners
------------------------------
Message: 7
Date: Tue, 10 Mar 2015 20:43:38 +0000
From: "Hunter, Theresa" <thunter <@t> hmc.psu.edu>
Subject: [Histonet] Job opportunity
To: "'histonet <@t> lists.utsouthwestern.edu'"
<histonet <@t> lists.utsouthwestern.edu>
Message-ID:
<38012F015BD58743B1EF391E522AD9B2BC025597 <@t> EXMBX4.mshmc.local>
Content-Type: text/plain; charset="us-ascii"
Hello everyone and happy National Histotechnologist Day!
We are looking for a Histology Supervisor to lead a great team in Hershey Pa. If you are interested, please follow the link below.
Thanks for your time,
Theresa
Career Website: http://www.pennstatehershey.org/web/humanresources/home/searchjobs
The Penn State Milton S. Hershey Medical Center is committed to affirmative action, equal opportunity and the diversity of its workforce. Equal Opportunity Employer - Minorities/Women/Protected Veterans/Disabled. All individuals (including current employees) selected for a position will undergo a background check appropriate for the position's responsibilities
Theresa Hunter, MLS(ASCP)cm
Interim Assistant Manager AP
Cytology/Decedent care/Histology
Lead Pathologist Assistant
Department of Pathology and Laboratory Medicine
Penn State Milton S. Hershey Medical Center
500 University Drive, Mail Code H160
Hershey, Pa. 17033
Phone (717) 531-0003 Ext. 287181
Fax (717)531-0831
thunter <@t> hmc.psu.edu<mailto:thunter <@t> hmc.psu.edu>
------------------------------
Message: 8
Date: Tue, 10 Mar 2015 20:50:27 +0000 (UTC)
From: victor_tobias <@t> comcast.net
Subject: [Histonet] TDP43, PTG poly
To: histonet <@t> lists.utsouthwestern.edu
Message-ID:
<680449490.1325591.1426020627172.JavaMail.zimbra <@t> comcast.net>
Content-Type: text/plain; charset=utf-8
We stain TDP43 on both FFPE and frozen sections. The FFPE are done on the Bond, but we can't seem to get as good results with the frozen sections on the Bond. Our neuropathologist still prefers manually stained frozen sections for TDP43 over ones done on the Bond. We would really like to get it working reliably on the Bond. ??Does anyone have a protocol they would be willing to share for frozen sections on the Bond?
Victor
University of Washington Medical Center
------------------------------
Message: 9
Date: Wed, 11 Mar 2015 09:21:08 +0900
From: Rui TAHARA <ruio7 <@t> hotmail.com>
Subject: RE: [Histonet] Re: Bird head stored in 70% alcohol and
possible decalcification
To: "histonet <@t> lists.utsouthwestern.edu"
<histonet <@t> lists.utsouthwestern.edu>
Message-ID: <BAY180-W46D4CD33AB4251D9B6F71CF9190 <@t> phx.gbl>
Content-Type: text/plain; charset="iso-2022-jp"
Thank you for all the helpful suggestions about this topic.
My sample has been in the fixative until it would be decalcified.
Thank you again,
rui
> From: ree3 <@t> leicester.ac.uk
> To: ruio7 <@t> hotmail.com
> Subject: RE: [Histonet] Re: Bird head stored in 70% alcohol and possible decalcification
> Date: Tue, 10 Mar 2015 09:49:00 +0000
>
> Leave it in formalin for as long as possible..........................good luck
>
> -----Original Message-----
> From: histonet-bounces <@t> lists.utsouthwestern.edu [mailto:histonet-bounces <@t> lists.utsouthwestern.edu] On Behalf Of Rui TAHARA
> Sent: 04 March 2015 23:39
> To: gayle.callis <@t> bresnan.net; histonet <@t> lists.utsouthwestern.edu
> Subject: RE: [Histonet] Re: Bird head stored in 70% alcohol and possible decalcification
>
> Thank you for helpful suggestions.
> I have further questions.
> Yes, I have a bird head (probably 1 cm X 1 cm ) stored in 70 % ethanol.
> But i have a similar size bird head fixed in 3.7% formalin for over night and am actually processing the head to store in 70% ethanol since my lab is just ordering the decalcifying solution. I need to decalcify this sample later.
> But i am wondering if it is better to keep the sample in formalin for a week or so till i get the decalcification solution or i should store it in 70 % ethanol and then fix it for a few days again later?
> I am afraid that longer fixative time would affect the sample somehow (e.g. the sample become too rigid?)
>
> Thank you,
>
> rui
>
> > From: gayle.callis <@t> bresnan.net
> > To: histonet <@t> lists.utsouthwestern.edu
> > Date: Wed, 4 Mar 2015 16:25:28 -0700
> > Subject: [Histonet] Re: Bird head stored in 70% alcohol and possible decalcification
> >
> > You wrote:
> >
> >
> >
> > I have an adult bird skull that fixed with formalin and then has been
> > stored in 70% ethanol.
> >
> > I have seen the post that the sample stored in 70% ethanol can be
> > walking back through to series of ethanol to water and can be
> > decalcified if it needs to be.
> >
> >
> >
> > I am wondering if anybody has done this and there is any side effects
> > from decalcification after going through dehydration and rehydration
> > of a sample compared to a general straight forward protocol from
> > decalcification to dehydration?
> >
> > **********************************************************************
> > ******
> > **********************************************************************
> > ******
> > ********************************
> >
> >
> >
> > I have, in the past, when a weekend arrive, I interrupted acid bone
> > decalcification by removing it from acid decalcifier, a quick water
> > rinse and immersed into 70% alcohol before returning bone to fresh
> > acid decalcifier the next working day. The bones always decalcified
> > without problems but I am sure the decalcification took longer since
> > partially decalcified bone had to rehydrate. I later learned more
> > about dipolar (hope I said that correctly) alcohol slowing and/or stopping ionization of calcium
> > and ceased using 70% alcohol to interrupt acid decalcification. I now use
> > NBF to interrupt decalcification. Interestingly, I learned the alcohol
> > technique from the AFIP bone pathology lab.
> >
> >
> >
> > Alcohol is put into Perenyi's nitric acid decalcifying solutions to slow
> > down or control very rapid nitric acid decalcification.
> >
> >
> >
> > You did not say how big the bird skull was? I suggest immersing the skull
> > back into NBF to let it totally rehydrate for several days (depending
> > on skull size and if the brain is present). I suggest changing NBF if you
> > rehydrate longer than a day. You don't need to go back through an alcohol
> > gradient since many processing schedules have tissue samples going from NBF
> > directly into 70%. If you leave residual alcohol in the bones, the acid
> > decalcification could be slower and hopefully not retarded in any way.
> >
> >
> >
> >
> > It certainly is worth a try. Good luck.
> >
> >
> >
> > Gayle M. Callis
> >
> > HTL/HT/MT(ASCP)
> >
> >
> >
> >
> >
> > _______________________________________________
> > Histonet mailing list
> > Histonet <@t> lists.utsouthwestern.edu
> > http://lists.utsouthwestern.edu/mailman/listinfo/histonet
>
------------------------------
Message: 10
Date: Tue, 10 Mar 2015 16:39:35 +0000 (UTC)
From: David Kemler <histotalk <@t> yahoo.com>
Subject: [Histonet] BIG day today!
To: Histonet <histonet <@t> lists.utsouthwestern.edu>
Message-ID:
<1358663623.2768352.1426005575141.JavaMail.yahoo <@t> mail.yahoo.com>
Content-Type: text/plain; charset=UTF-8
That's right, it's OUR day!
NSH is doing their thing. Link here: http://www.nsh.org/content/histotechnology-professionals-day .
Yours,Dave
------------------------------
Message: 11
Date: Tue, 10 Mar 2015 23:47:25 -0400
From: Mike <mikeykkk_3 <@t> live.com>
Subject: [Histonet] PA
To: "Histonet <@t> lists.utsouthwestern.edu"
<Histonet <@t> lists.utsouthwestern.edu>
Message-ID: <BLU436-SMTP16351CFBC48A0B1EF5EF0A9D7190 <@t> phx.gbl>
Content-Type: text/plain; charset="us-ascii"
Can you be a pathology assistant with on the job training only? Does it matter if you have a bachelors vs a masters degree in a science not related to pathology or a tech license vs no tech license?
Thanks
Sent from my iPhone
------------------------------
Message: 12
Date: Tue, 10 Mar 2015 21:17:05 -0700
From: Jennifer MacDonald <JMacDonald <@t> mtsac.edu>
Subject: Re: [Histonet] PA
To: Mike <mikeykkk_3 <@t> live.com>
Cc: "Histonet <@t> lists.utsouthwestern.edu"
<Histonet <@t> lists.utsouthwestern.edu>,
histonet-bounces <@t> lists.utsouthwestern.edu
Message-ID:
<OF4C2A119D.CCC4F1AB-ON88257E05.0015E2A0-88257E05.00177C1B <@t> mtsac.edu>
Content-Type: text/plain; charset="UTF-8"
To be a PA you would need to have ASCP certification. There is one route
to be eligible for certification.
To be eligible for this examination category, an applicant must have a
minimum of a baccalaureate degree from a regionally accredited
college/university, AND successful completion of a NAACLS accredited
Pathologists??? Assistant program within the last five years.
Most of the NAACLS programs are Masters degrees.
From: Mike <mikeykkk_3 <@t> live.com>
To: "Histonet <@t> lists.utsouthwestern.edu"
<Histonet <@t> lists.utsouthwestern.edu>
Date: 03/10/2015 08:49 PM
Subject: [Histonet] PA
Sent by: histonet-bounces <@t> lists.utsouthwestern.edu
Can you be a pathology assistant with on the job training only? Does it
matter if you have a bachelors vs a masters degree in a science not
related to pathology or a tech license vs no tech license?
Thanks
Sent from my iPhone
_______________________________________________
Histonet mailing list
Histonet <@t> lists.utsouthwestern.edu
http://lists.utsouthwestern.edu/mailman/listinfo/histonet
------------------------------
Message: 13
Date: Wed, 11 Mar 2015 12:49:32 +0000
From: Nancy Schmitt <Nancy_Schmitt <@t> pa-ucl.com>
Subject: [Histonet] Diff Quik troubleshooting
To: "'histonet <@t> lists.utsouthwestern.edu'"
<histonet <@t> lists.utsouthwestern.edu>
Message-ID:
<906B4DA90ED1DB4DB6C7E94D7CEE6C3601159B7F92 <@t> PEITHA.wad.pa-ucl.com>
Content-Type: text/plain; charset="us-ascii"
Good Morning-
Random FNA case where the slides turn blue as they dry. We have tried taking them back through stains, destaining and restaining, etc. FNA smears are air-dried when we receive them on plain slides, unfixed.
Any thoughts appreciated.
Nancy Schmitt MLT, HT(ASCP)
United Clinical Laboratories
Dubuque, IA
NOTICE: This email may contain legally privileged information. The information
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attachments. Thank you.
------------------------------
Message: 14
Date: Wed, 11 Mar 2015 08:52:05 -0400
From: Klaus Dern <klaus.dern44 <@t> gmail.com>
Subject: [Histonet] THICK AND THIN SECTIONS ?
To: histonet <histonet <@t> lists.utsouthwestern.edu>
Message-ID:
<CAJhryisZAh9wS1MokgaT70LhoUh39_SqZYauW0Sovj8_sm_t=A <@t> mail.gmail.com>
Content-Type: text/plain; charset=UTF-8
If you are using one of the following microtomes and are being told the
advance mechanism is worn out. ( too much play between spindle and spindle
nut )
You could be faced with purchasing a new Microtome. ( No parts
availability )
REICHERT/JUNG 2030
LEICA RM 2125
LEICA 2030 Biocut
LEICA/JUNG 2035
LEICA - CM 1850 Cryostat
SAKURA SRM 200
Rather than replacing these excellent Instruments, I have a PERMANENT
solution to fix this problem.
For Information, contact:
Klaus Dern
Phone: 706 635-8840
E-Mail: klaus.dern44 <@t> gmail.com
------------------------------
Message: 15
Date: Wed, 11 Mar 2015 15:42:45 +0000
From: "Mayer,Toysha N" <TNMayer <@t> mdanderson.org>
Subject: [Histonet] RE: Old slides
To: "'histonet <@t> lists.utsouthwestern.edu'"
<histonet <@t> lists.utsouthwestern.edu>
Message-ID:
<47E9B2C01DDDD94881EACD2DC44EBC881D5082D7 <@t> D1PWPEXMBX05.mdanderson.edu>
Content-Type: text/plain; charset="us-ascii"
Bernice,
Take the slide and dip it in xylene. Lay it on the film, pressing down firmly. As it adheres, then gently wipe the excess xylene off, and gently place it in a book or your procedure manual and leave it there for an hour or so.
Most of the bubbles will be gone, and the tissue will be saved.
The original problem is not enough xylene dispersed onto the slide. Adjust the flow being dispensed by the unit.
Sincerely,
Toysha N. Mayer, D.H.Sc., MBA, HT (ASCP)
Instructor/Education Coordinator
Program in Histotechnology
School of Health Professions
UT M.D. Anderson Cancer Center
713.563-3481
----------------------------------------------------------------------
Message: 1
Date: Mon, 9 Mar 2015 19:41:48 +0000
From: Bernice Frederick <b-frederick <@t> northwestern.edu>
Subject: [Histonet] Old slides.
To: "histonet <@t> lists.utsouthwestern.edu"
<histonet <@t> lists.utsouthwestern.edu>
Message-ID:
<eb9d7062461640e5ac22d213a4acc16f <@t> evcspmbx03.ads.northwestern.edu>
Content-Type: text/plain; charset="us-ascii"
Hi all,
We received some old slides (1997-1998) that were coverslipped with film. Sakura I would imagine. The issue here is that the coverslips have come up from the slide and the tissue is adhered to the back of the coverslip. They need to be recovered so they can be evaluated. What do you all recommend? We use the CV5030 for coverslipping. I tried one with xylene and mounting media but there were still a couple of air bubbles in there.
Thanks,
Bernice
Bernice Frederick HTL (ASCP)
Senior Research Tech
Pathology Core Facility
Robert. H. Lurie Cancer Center
Northwestern University
710 N Fairbanks Court
Olson 8-421
Chicago,IL 60611
312-503-3723
b-frederick <@t> northwestern.edu<mailto:b-frederick <@t> northwestern.edu>
------------------------------
Message: 16
Date: Wed, 11 Mar 2015 12:53:13 -0300
From: Mariela Chertoff <marielachertoff <@t> gmail.com>
Subject: [Histonet] 5-methylcytosine IHC in tissue
To: histonet <@t> lists.utsouthwestern.edu
Message-ID:
<CAC6nBUvyuxafo2tm-+A28+=hb506YdU3kOq4KppVPYq7VuL6Hg <@t> mail.gmail.com>
Content-Type: text/plain; charset=UTF-8
Hi
Has anybody experience with 5-metcyt staining in brain tissue? some advice
about the HCl and Boric acid treatment?
I use 50um free floating sections ferfused with PFA 4%.
Thank you in advance for your help!!
Mariela Chertoff, PhD
Laboratorio de Neuroepigenetica - QB75
Departamento de Qu??mica Biol??gica
Facultad de Ciencias Exactas y Naturales - UBA
Ciudad Universitaria Pabell??n II Piso 4
Ciudad Aut??noma de Buenos Aires
C1428EGA - Argentina
Tel: 54 11 4576-3300/09 - Int. 221
email:marielachertoff <@t> gmail.com
marielachertoff <@t> qb.fcen.uba.ar
<mariela.chertoff <@t> uab.cat>
------------------------------
Message: 17
Date: Wed, 11 Mar 2015 15:53:12 +0000
From: "Mayer,Toysha N" <TNMayer <@t> mdanderson.org>
Subject: [Histonet] RE: Masson Trichrome stain
To: "'histonet <@t> lists.utsouthwestern.edu'"
<histonet <@t> lists.utsouthwestern.edu>
Message-ID:
<47E9B2C01DDDD94881EACD2DC44EBC881D508317 <@t> D1PWPEXMBX05.mdanderson.edu>
Content-Type: text/plain; charset="us-ascii"
Justine,
I do not have any metal forceps in the special stains area, due to the reaction that they can cause when staining with silver. As a rule of thumb, it is just easier to use plastic all the way around.
The Carson text does not state the use of only plastic forceps, but I would think that maybe they are concerned with a reaction between the Weigert's and the metal. That would be a stretch.
As for no water before aniline blue, I believe the concentration is very weak and the water may dilute they dye even further. This would affect the staining results.
Sincerely,
Toysha N. Mayer, D.H.Sc., MBA, HT (ASCP)
Instructor/Education Coordinator
Program in Histotechnology
School of Health Professions
UT M.D. Anderson Cancer Center
713.563-3481
------------------------------
Message: 4
Date: Tue, 10 Mar 2015 00:31:56 -0500
From: John Kiernan <jkiernan <@t> uwo.ca>
Subject: Re: [Histonet] FW: Masson's trichrome stain
To: Linda Margraf <lindamargraf <@t> gmail.com>,
histonet <@t> lists.utsouthwestern.edu
Cc: justinelanzon <@t> hotmail.com
Message-ID: <7380eaed48941.54fe3b7c <@t> uwo.ca>
Content-Type: text/plain; charset=iso-8859-1
The notion of plastic forceps is new to me. Where did Justine find it? Nothing in any variant of the Masson procedure should be adversely affected by moving slides with stainless steel forceps. Is there a commercial campaign to sell plastic tweezers to Histonetters?
John Kiernan
= = =
On 08/03/15, Linda Margraf <lindamargraf <@t> gmail.com> wrote:
> Here is a message from Justine...
>
> From: Justine Lanzon [mailto:justinelanzon <@t> hotmail.com]
> <justinelanzon <@t> hotmail.com]>
> Sent: Thursday, March 05, 2015 5:36 AM
> To: lindamargraf <@t> gmail.com
> Subject: Masson's trichrome stain
>
>
> Hi,
>
> I am doing a write up on Masson's trichrome stain however I cannot
> answer these two questions:
>
> - Why are plastic forceps used instead of metal ones to hold the
> stained slide?
>
> - Why do we not rinse before Alinine blue?
>
> ?
>
> Can you please help me?
>
> ?
>
> Many Thanks,
>
> Justine Lanzon
>
> _______________________________________________
> Histonet mailing list
> Histonet <@t> lists.utsouthwestern.edu
> http://lists.utsouthwestern.edu/mailman/listinfo/histonet
>
>
------------------------------
------------------------------
Message: 18
Date: Wed, 11 Mar 2015 16:04:35 +0000
From: "Marcum, Pamela A" <PAMarcum <@t> uams.edu>
Subject: [Histonet] RE: Old slides
To: "'Mayer,Toysha N'" <TNMayer <@t> mdanderson.org>,
"'histonet <@t> lists.utsouthwestern.edu'"
<histonet <@t> lists.utsouthwestern.edu>
Message-ID: <8cf69427ea8f4cdf94fc08543f2d42df <@t> MAIL13M2N2.ad.uams.edu>
Content-Type: text/plain; charset="us-ascii"
We have literally about one hundred slides to re-slip for the this reason. Are there any suggestions for large numbers of slides to be re-coverslipped as this method would be too time consuming. We have used only glass for about nine years or so and it is much better. The old ones are the problem when someone needs "THAT" slide only.
Pam Marcum
UAMS
-----Original Message-----
From: histonet-bounces <@t> lists.utsouthwestern.edu [mailto:histonet-bounces <@t> lists.utsouthwestern.edu] On Behalf Of Mayer,Toysha N
Sent: Wednesday, March 11, 2015 10:43 AM
To: 'histonet <@t> lists.utsouthwestern.edu'
Subject: [Histonet] RE: Old slides
Bernice,
Take the slide and dip it in xylene. Lay it on the film, pressing down firmly. As it adheres, then gently wipe the excess xylene off, and gently place it in a book or your procedure manual and leave it there for an hour or so.
Most of the bubbles will be gone, and the tissue will be saved.
The original problem is not enough xylene dispersed onto the slide. Adjust the flow being dispensed by the unit.
Sincerely,
Toysha N. Mayer, D.H.Sc., MBA, HT (ASCP) Instructor/Education Coordinator Program in Histotechnology School of Health Professions UT M.D. Anderson Cancer Center
713.563-3481
----------------------------------------------------------------------
Message: 1
Date: Mon, 9 Mar 2015 19:41:48 +0000
From: Bernice Frederick <b-frederick <@t> northwestern.edu>
Subject: [Histonet] Old slides.
To: "histonet <@t> lists.utsouthwestern.edu"
<histonet <@t> lists.utsouthwestern.edu>
Message-ID:
<eb9d7062461640e5ac22d213a4acc16f <@t> evcspmbx03.ads.northwestern.edu>
Content-Type: text/plain; charset="us-ascii"
Hi all,
We received some old slides (1997-1998) that were coverslipped with film. Sakura I would imagine. The issue here is that the coverslips have come up from the slide and the tissue is adhered to the back of the coverslip. They need to be recovered so they can be evaluated. What do you all recommend? We use the CV5030 for coverslipping. I tried one with xylene and mounting media but there were still a couple of air bubbles in there.
Thanks,
Bernice
Bernice Frederick HTL (ASCP)
Senior Research Tech
Pathology Core Facility
Robert. H. Lurie Cancer Center
Northwestern University
710 N Fairbanks Court
Olson 8-421
Chicago,IL 60611
312-503-3723
b-frederick <@t> northwestern.edu<mailto:b-frederick <@t> northwestern.edu>
_______________________________________________
Histonet mailing list
Histonet <@t> lists.utsouthwestern.edu
http://lists.utsouthwestern.edu/mailman/listinfo/histonet
----------------------------------------------------------------------
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------------------------------
Message: 19
Date: Wed, 11 Mar 2015 16:52:22 +0000
From: "Solis, Bryan" <BSolis <@t> Fibrogen.com>
Subject: [Histonet] RE: Histonet Digest, Vol 136, Issue 12
To: "'histonet <@t> lists.utsouthwestern.edu'"
<histonet <@t> lists.utsouthwestern.edu>
Message-ID: <8c222bc2cfb24928808faf2d0622820a <@t> SF1EX007.fibrogen.com>
Content-Type: text/plain; charset="us-ascii"
Hello,
We received some liver tissue (Mouse) in 10% formalin (NFB). Then transferred to 70% ETOH. My question is that, Is it ok to transfer to 30% sucrose? So, frozen section can be perform. Please advise.
Thanks,
Bryan S.
-----Original Message-----
From: histonet-bounces <@t> lists.utsouthwestern.edu [mailto:histonet-bounces <@t> lists.utsouthwestern.edu] On Behalf Of histonet-request <@t> lists.utsouthwestern.edu
Sent: Tuesday, March 10, 2015 10:06 AM
To: histonet <@t> lists.utsouthwestern.edu
Subject: Histonet Digest, Vol 136, Issue 12
Send Histonet mailing list submissions to
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To subscribe or unsubscribe via the World Wide Web, visit
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or, via email, send a message with subject or body 'help' to
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When replying, please edit your Subject line so it is more specific than "Re: Contents of Histonet digest..."
Today's Topics:
1. Old slides. (Bernice Frederick)
2. RE: Old slides. (Jason McGough)
3. RE: Mushrooms for GMS fungus control (Morken, Timothy)
4. Re: FW: Masson's trichrome stain (John Kiernan)
5. RE: Old slides. (John Kiernan)
6. soft for microwriter (thermo scientific, Lamb, Shandon)
(richard wild)
7. Re: Old slides. (b.curran.mcwilliam <@t> gmail.com)
8. Re: Old slides. (Rene J Buesa)
9. RE: Old slides. (Gowan,Christie C)
10. IHC / Morphometry Technician wanted in Shenandoah Valley
Virginia (Erin Sarricks)
----------------------------------------------------------------------
Message: 1
Date: Mon, 9 Mar 2015 19:41:48 +0000
From: Bernice Frederick <b-frederick <@t> northwestern.edu>
Subject: [Histonet] Old slides.
To: "histonet <@t> lists.utsouthwestern.edu"
<histonet <@t> lists.utsouthwestern.edu>
Message-ID:
<eb9d7062461640e5ac22d213a4acc16f <@t> evcspmbx03.ads.northwestern.edu>
Content-Type: text/plain; charset="us-ascii"
Hi all,
We received some old slides (1997-1998) that were coverslipped with film. Sakura I would imagine. The issue here is that the coverslips have come up from the slide and the tissue is adhered to the back of the coverslip. They need to be recovered so they can be evaluated. What do you all recommend? We use the CV5030 for coverslipping. I tried one with xylene and mounting media but there were still a couple of air bubbles in there.
Thanks,
Bernice
Bernice Frederick HTL (ASCP)
Senior Research Tech
Pathology Core Facility
Robert. H. Lurie Cancer Center
Northwestern University
710 N Fairbanks Court
Olson 8-421
Chicago,IL 60611
312-503-3723
b-frederick <@t> northwestern.edu<mailto:b-frederick <@t> northwestern.edu>
------------------------------
Message: 2
Date: Mon, 9 Mar 2015 14:20:09 -0600
From: Jason McGough <jmcgough <@t> clinlab.com>
Subject: RE: [Histonet] Old slides.
To: Bernice Frederick <b-frederick <@t> northwestern.edu>,
histonet <@t> lists.utsouthwestern.edu <histonet <@t> lists.utsouthwestern.edu>
Message-ID: <zarafa.54fe0079.5165.2525b65b03ddef5e <@t> mail.clinlab.com>
Content-Type: text/plain; charset=utf-8
Remove the film coverslip by placing the slide in acetone for a few minutes. Then recoverslip the slide with your current method.
Jason McGough, HT(ASCP)
Operations Manager
Clinical Laboratory of the Black Hills
605-343-2267
jmcgough <@t> clinlab.com <mailto:jmcgough <@t> clinlab.com>
www.clinlab.com <http://www.clinlab.com>
-----Original message-----
> From:Bernice Frederick <b-frederick <@t> northwestern.edu
> <mailto:b-frederick <@t> northwestern.edu> >
> Sent: Monday, March 9, 2015 1:51 PM
> To: histonet <@t> lists.utsouthwestern.edu
> <mailto:histonet <@t> lists.utsouthwestern.edu>
> Subject: [Histonet] Old slides.
>
> Hi all,
> We received some old slides (1997-1998) that were coverslipped with film. Sakura I would imagine. The issue here is that the coverslips have come up from the slide and the tissue is adhered to the back of the coverslip. They need to be recovered so they can be evaluated. What do you all recommend? We use the CV5030 for coverslipping. I tried one with xylene and mounting media but there were still a couple of air bubbles in there.
> Thanks,
> Bernice
>
> Bernice Frederick HTL (ASCP)
> Senior Research Tech
> Pathology Core Facility
> Robert. H. Lurie Cancer Center
> Northwestern University
> 710 N Fairbanks Court
> Olson 8-421
> Chicago,IL 60611
> 312-503-3723
> b-frederick <@t> northwestern.edu <mailto:b-frederick <@t> northwestern.edu>
> <mailto:b-frederick <@t> northwestern.edu
> <mailto:b-frederick <@t> northwestern.edu> >
>
> _______________________________________________
> Histonet mailing list
> Histonet <@t> lists.utsouthwestern.edu
> <mailto:Histonet <@t> lists.utsouthwestern.edu>
> http://lists.utsouthwestern.edu/mailman/listinfo/histonet
> <http://lists.utsouthwestern.edu/mailman/listinfo/histonet>
>
>
------------------------------
Message: 3
Date: Mon, 9 Mar 2015 22:46:08 +0000
From: "Morken, Timothy" <Timothy.Morken <@t> ucsf.edu>
Subject: [Histonet] RE: Mushrooms for GMS fungus control
To: "koellingr <@t> comcast.net" <koellingr <@t> comcast.net>
Cc: "histonet <@t> lists.utsouthwestern.edu"
<histonet <@t> lists.utsouthwestern.edu>
Message-ID:
<761E2B5697F795489C8710BCC72141FF367FBA31 <@t> ex07.net.ucsf.edu>
Content-Type: text/plain; charset="utf-8"
Try this article...
Acta Cytol. 2003 Nov-Dec;47(6):1043-4.
Alternative, cost-effective fungus-staining method for control slides in cytology and histopathology.
da Silva VD1.
Author information
Abstract
OBJECTIVE:
To develop a cost-effective, reliable and safe method of providing fungal control slides for routine use in pathology laboratories.
STUDY DESIGN:
A set of easily available, low-cost material was tested to obtain fungal colonies on substrate adequate for paraffin-embedded sections or smears.
RESULTS:
Such material as cheese is a simple, inexpensive and practical culture medium for silver-positive fungi. A batch of paraffin blocks can be prepared to maintain a stock of control material in the laboratory.
CONCLUSION:
It is useful to maintain fungal colonies to produce staining control specimens using small pieces of refrigerated cheese to easily produce silver-staining control specimens or smears embedded in paraffin, reducing the risk of accidental exposure to potentially infective pathogens in the laboratory. This method might also be a good alternative for conserving routine surgical specimens, considering the currently decreasing numbers of necropsy and large specimens, particularly from immunosuppressed and infected patients.
PMID: 14674076 [PubMed - indexed for MEDLINE]
-----Original Message-----
From: histonet-bounces <@t> lists.utsouthwestern.edu [mailto:histonet-bounces <@t> lists.utsouthwestern.edu] On Behalf Of koellingr <@t> comcast.net
Sent: Sunday, March 08, 2015 4:29 PM
To: Linda Prasad (SCHN)
Cc: Jeffrey Robinson; histonet <@t> lists.utsouthwestern.edu
Subject: Re: [Histonet] RE: Mushrooms for GMS fungus control
Apparently there are numerous interesting ways for fungus or bacteria controls to be had from orange peels to hamburger to slim Jim's to hot dogs to strawberries to ????.?? Sounds like fun to me.?? I'm curious, with the emphasis now on quality control in labs??run amok, has anyone passed a rigorous inspection actually showing these as your currently in-use controls??? A PI in research who??doesn't want??his paper rejected at peer review.?? A CAP inspector in clinical labs who is nit-picky reviewing staining controls but might be looking for a phase anything deficiency.?? The??dot-your-i's and cross-your-t's??FDA people who might or might not OK your drug in development.?? Really, just curious if anyone with a hammer over your head has said it is perfectly fine to use them.
Ray, Seattle, WA
----- Original Message -----
From: "Linda Prasad (SCHN)" <linda.prasad <@t> health.nsw.gov.au>
To: "Jeffrey Robinson" <JRobinson <@t> pathology-associates.com>, histonet <@t> lists.utsouthwestern.edu
Sent: Sunday, March 8, 2015 4:09:02 PM
Subject: [Histonet] RE: Mushrooms for GMS fungus control
I used strawberries for a fungal control. Worked really good.
Linda Prasad | Senior Scientist | Histopathology
t: (02) 9845 3306 | f: (02) 9845 3318 | e: linda.prasad <@t> health.nsw.gov.au | w: www.schn.health.nsw.gov.au
Cnr Hawkesbury Road and Hainsworth Street, Westmead, NSW Australia Locked Bag 4001, Westmead 2145, NSW Australia
???????Please consider the environment before printing this email.
-----Original Message-----
From: histonet-bounces <@t> lists.utsouthwestern.edu [mailto:histonet-bounces <@t> lists.utsouthwestern.edu] On Behalf Of Jeffrey Robinson
Sent: Saturday, 7 March 2015 4:16 AM
To: histonet <@t> lists.utsouthwestern.edu
Subject: [Histonet] Mushrooms for GMS fungus control
How about mushrooms? ??Has anyone had any success using mushrooms as a GMS fungus control?
Jeff Robinson, Senior Histotechnologist, Sierra Pathology Lab, Clovis, CA
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------------------------------
Message: 4
Date: Tue, 10 Mar 2015 00:31:56 -0500
From: John Kiernan <jkiernan <@t> uwo.ca>
Subject: Re: [Histonet] FW: Masson's trichrome stain
To: Linda Margraf <lindamargraf <@t> gmail.com>,
histonet <@t> lists.utsouthwestern.edu
Cc: justinelanzon <@t> hotmail.com
Message-ID: <7380eaed48941.54fe3b7c <@t> uwo.ca>
Content-Type: text/plain; charset=iso-8859-1
The notion of plastic forceps is new to me. Where did Justine find it? Nothing in any variant of the Masson procedure should be adversely affected by moving slides with stainless steel forceps. Is there a commercial campaign to sell plastic tweezers to Histonetters?
John Kiernan
= = =
On 08/03/15, Linda Margraf <lindamargraf <@t> gmail.com> wrote:
> Here is a message from Justine...
>
> From: Justine Lanzon [mailto:justinelanzon <@t> hotmail.com]
> <justinelanzon <@t> hotmail.com]>
> Sent: Thursday, March 05, 2015 5:36 AM
> To: lindamargraf <@t> gmail.com
> Subject: Masson's trichrome stain
>
>
> Hi,
>
> I am doing a write up on Masson's trichrome stain however I cannot
> answer these two questions:
>
> - Why are plastic forceps used instead of metal ones to hold the
> stained slide?
>
> - Why do we not rinse before Alinine blue?
>
> ?
>
> Can you please help me?
>
> ?
>
> Many Thanks,
>
> Justine Lanzon
>
> _______________________________________________
> Histonet mailing list
> Histonet <@t> lists.utsouthwestern.edu
> http://lists.utsouthwestern.edu/mailman/listinfo/histonet
>
>
------------------------------
Message: 5
Date: Tue, 10 Mar 2015 00:40:02 -0500
From: John Kiernan <jkiernan <@t> uwo.ca>
Subject: RE: [Histonet] Old slides.
To: Jason McGough <jmcgough <@t> clinlab.com>, Bernice Frederick
<b-frederick <@t> northwestern.edu>, histonet <@t> lists.utsouthwestern.edu,
histonet <@t> lists.utsouthwestern.edu
Message-ID: <73e09aa74b442.54fe3d62 <@t> uwo.ca>
Content-Type: text/plain; charset=iso-8859-1
Have you done this? Acetone does not dissolve resinous mounting media and allow removal of coverslips. It's all in the books; buy one.
John Kiernan
Anatomy & Cell Biology, UWO
London, Canada
= = =
On 09/03/15, Jason McGough <jmcgough <@t> clinlab.com> wrote:
> Remove the film coverslip by placing the slide in acetone for a few minutes. Then recoverslip the slide with your current method.
>
>
>
> Jason McGough, HT(ASCP)
>
> Operations Manager
>
> Clinical Laboratory of the Black Hills
>
> 605-343-2267
>
> jmcgough <@t> clinlab.com <mailto:jmcgough <@t> clinlab.com
> <jmcgough <@t> clinlab.com>>
>
> www.clinlab.com <http://www.clinlab.com>
>
> ?
> ?
> -----Original message-----
> > From:Bernice Frederick <b-frederick <@t> northwestern.edu
> > <mailto:b-frederick <@t> northwestern.edu <b-frederick <@t> northwestern.edu>>
> > >
> > Sent: Monday, March 9, 2015 1:51 PM
> > To: histonet <@t> lists.utsouthwestern.edu
> > <mailto:histonet <@t> lists.utsouthwestern.edu
> > <histonet <@t> lists.utsouthwestern.edu>>
> > Subject: [Histonet] Old slides.
> >
> > Hi all,
> > We received some old slides (1997-1998) that were coverslipped with film. Sakura I would imagine. The issue here is that the coverslips have come up from the slide and the tissue is adhered to the back of the coverslip. They need to be recovered so they can be evaluated. What do you all recommend? We use the CV5030 for coverslipping. I tried one with xylene and mounting media but there were still a couple of air bubbles in there.
> > Thanks,
> > Bernice
> >
> > Bernice Frederick HTL (ASCP)
> > Senior Research Tech
> > Pathology Core Facility
> > Robert. H. Lurie Cancer Center
> > Northwestern University
> > 710 N Fairbanks Court
> > Olson 8-421
> > Chicago,IL 60611
> > 312-503-3723
> > b-frederick <@t> northwestern.edu <mailto:b-frederick <@t> northwestern.edu
> > <b-frederick <@t> northwestern.edu>> <mailto:b-frederick <@t> northwestern.edu
> > <b-frederick <@t> northwestern.edu> <mailto:b-frederick <@t> northwestern.edu
> > <b-frederick <@t> northwestern.edu>> >
> >
> > _______________________________________________
> > Histonet mailing list
> > Histonet <@t> lists.utsouthwestern.edu
> > <mailto:Histonet <@t> lists.utsouthwestern.edu
> > <Histonet <@t> lists.utsouthwestern.edu>>
> > http://lists.utsouthwestern.edu/mailman/listinfo/histonet
> > <http://lists.utsouthwestern.edu/mailman/listinfo/histonet>
> >
> >
>
>
> _______________________________________________
> Histonet mailing list
> Histonet <@t> lists.utsouthwestern.edu
> http://lists.utsouthwestern.edu/mailman/listinfo/histonet
>
>
------------------------------
Message: 6
Date: Tue, 10 Mar 2015 10:05:11 +0100
From: richard wild <richard.wild <@t> wanadoo.fr>
Subject: [Histonet] soft for microwriter (thermo scientific, Lamb,
Shandon)
To: histonet <@t> lists.utsouthwestern.edu
Message-ID: <54FEB3C7.40306 <@t> wanadoo.fr>
Content-Type: text/plain; charset=utf-8; format=flowed
Hi
I am looking for labelling software (or advices) for the carousel microwriter (thermo scientific, Lamb, Shandon = same machine) (LAMB E22.01MWR) The machine is discontinuated.
I would like to use serial interface rs232 and barcode scanners.
Thanks for help.
Richard
------------------------------
Message: 7
Date: Tue, 10 Mar 2015 11:51:25 +0000
From: b.curran.mcwilliam <@t> gmail.com
Subject: Re: [Histonet] Old slides.
To: Bernice Frederick <b-frederick <@t> northwestern.edu>
Cc: "histonet <@t> lists.utsouthwestern.edu"
<histonet <@t> lists.utsouthwestern.edu>
Message-ID: <9FA4B5FB-11BE-47C7-AA0C-6F8416B10487 <@t> gmail.com>
Content-Type: text/plain; charset=us-ascii
Hi
We re-Coverslipper a number of sections which had peeled off on the tape as the tape dried and curled. We cut off excess tape using scissors; placed a fresh coverslip flat; put a streak of mounting medium on he coverslip; use a forceps to orientate the tissue + margin of tape; number a slide, dip it in Xylene & place on a slope & bring on top of coverslip-section-mounting medium; turn the slide-section- coverslip to face coverslip up; leave horizontal to dry (eg overnight). Worth a try, doing one first.
Bernie,
St Vincent's,
Dublin,
Ireland
> On 9 Mar 2015, at 19:41, Bernice Frederick <b-frederick <@t> northwestern.edu> wrote:
>
> Hi all,
> We received some old slides (1997-1998) that were coverslipped with film. Sakura I would imagine. The issue here is that the coverslips have come up from the slide and the tissue is adhered to the back of the coverslip. They need to be recovered so they can be evaluated. What do you all recommend? We use the CV5030 for coverslipping. I tried one with xylene and mounting media but there were still a couple of air bubbles in there.
> Thanks,
> Bernice
>
> Bernice Frederick HTL (ASCP)
> Senior Research Tech
> Pathology Core Facility
> Robert. H. Lurie Cancer Center
> Northwestern University
> 710 N Fairbanks Court
> Olson 8-421
> Chicago,IL 60611
> 312-503-3723
> b-frederick <@t> northwestern.edu<mailto:b-frederick <@t> northwestern.edu>
>
> _______________________________________________
> Histonet mailing list
> Histonet <@t> lists.utsouthwestern.edu
> http://lists.utsouthwestern.edu/mailman/listinfo/histonet
------------------------------
Message: 8
Date: Tue, 10 Mar 2015 13:43:35 +0000 (UTC)
From: Rene J Buesa <rjbuesa <@t> yahoo.com>
Subject: Re: [Histonet] Old slides.
To: John Kiernan <jkiernan <@t> uwo.ca>, Jason McGough
<jmcgough <@t> clinlab.com>, Bernice Frederick
<b-frederick <@t> northwestern.edu>, "histonet <@t> lists.utsouthwestern.edu"
<histonet <@t> lists.utsouthwestern.edu>
Message-ID:
<1897656965.1753669.1425995015431.JavaMail.yahoo <@t> mail.yahoo.com>
Content-Type: text/plain; charset=UTF-8
To John
Probably the writer is referring to slides "mounted" with the Sakura film??"coverslip".I have done it many times and the FILM will??detach easily from the section.Had it been a glass coverslip attached with, for example, Permount, acetone would not have done anything.Ren?? J.??
On Tuesday, March 10, 2015 1:40 AM, John Kiernan <jkiernan <@t> uwo.ca> wrote:
Have you done this? Acetone does not dissolve resinous mounting media and allow removal of coverslips. It's all in the books; buy one.
John Kiernan
Anatomy & Cell Biology, UWO
London, Canada
= = =
On 09/03/15, Jason McGough?? <jmcgough <@t> clinlab.com> wrote:
> Remove the film coverslip by placing the slide in acetone for a few minutes. Then recoverslip the slide with your current method.
>
>
>
> Jason McGough, HT(ASCP)
>
> Operations Manager
>
> Clinical Laboratory of the Black Hills
>
> 605-343-2267
>
> jmcgough <@t> clinlab.com <mailto:jmcgough <@t> clinlab.com <jmcgough <@t> clinlab.com>>
>
> www.clinlab.com <http://www.clinlab.com>
>
> ??
> ??
> -----Original message-----
> > From:Bernice Frederick <b-frederick <@t> northwestern.edu <mailto:b-frederick <@t> northwestern.edu <b-frederick <@t> northwestern.edu>> >
> > Sent: Monday, March 9, 2015 1:51 PM
> > To: histonet <@t> lists.utsouthwestern.edu <mailto:histonet <@t> lists.utsouthwestern.edu <histonet <@t> lists.utsouthwestern.edu>>
> > Subject: [Histonet] Old slides.
> >
> > Hi all,
> > We received some old slides (1997-1998) that were coverslipped with film. Sakura I would imagine. The issue here is that the coverslips have come up from the slide and the tissue is adhered to the back of the coverslip. They need to be recovered so they can be evaluated. What do you all recommend? We use the CV5030 for coverslipping. I tried one with xylene and mounting media but there were still a couple of air bubbles in there.
> > Thanks,
> > Bernice
> >
> > Bernice Frederick HTL (ASCP)
> > Senior Research Tech
> > Pathology Core Facility
> > Robert. H. Lurie Cancer Center
> > Northwestern University
> > 710 N Fairbanks Court
> > Olson 8-421
> > Chicago,IL 60611
> > 312-503-3723
> > b-frederick <@t> northwestern.edu <mailto:b-frederick <@t> northwestern.edu <b-frederick <@t> northwestern.edu>> <mailto:b-frederick <@t> northwestern.edu <b-frederick <@t> northwestern.edu> <mailto:b-frederick <@t> northwestern.edu <b-frederick <@t> northwestern.edu>> >
> >
> > _______________________________________________
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> > Histonet <@t> lists.utsouthwestern.edu <mailto:Histonet <@t> lists.utsouthwestern.edu <Histonet <@t> lists.utsouthwestern.edu>>
> > http://lists.utsouthwestern.edu/mailman/listinfo/histonet <http://lists.utsouthwestern.edu/mailman/listinfo/histonet>
> >
> >
>
>
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------------------------------
Message: 9
Date: Mon, 9 Mar 2015 20:01:10 +0000
From: "Gowan,Christie C" <christiecgowan <@t> dermatology.med.ufl.edu>
Subject: [Histonet] RE: Old slides.
To: "'Bernice Frederick'" <b-frederick <@t> northwestern.edu>,
"histonet <@t> lists.utsouthwestern.edu"
<histonet <@t> lists.utsouthwestern.edu>
Message-ID: <de45f62de02d4c3389de0af3f45e191a <@t> AHC-EXCH03.ad.ufl.edu>
Content-Type: text/plain; charset="us-ascii"
Hi Bernice,
I have found that if you flood the slide with mounting media (don't use xylene) flip the slide over onto an absorbent lab wipe and put a heavy weight with even pressure and leave for a few hours. If the slide sticks to the wipe just put a few drops of xylene to clean up the slide. You may still have some tiny bubbles but it is much better than the alternative. Good luck.
Christie Gowan
-----Original Message-----
From: histonet-bounces <@t> lists.utsouthwestern.edu [mailto:histonet-bounces <@t> lists.utsouthwestern.edu] On Behalf Of Bernice Frederick
Sent: Monday, March 09, 2015 3:42 PM
To: histonet <@t> lists.utsouthwestern.edu
Subject: [Histonet] Old slides.
Hi all,
We received some old slides (1997-1998) that were coverslipped with film. Sakura I would imagine. The issue here is that the coverslips have come up from the slide and the tissue is adhered to the back of the coverslip. They need to be recovered so they can be evaluated. What do you all recommend? We use the CV5030 for coverslipping. I tried one with xylene and mounting media but there were still a couple of air bubbles in there.
Thanks,
Bernice
Bernice Frederick HTL (ASCP)
Senior Research Tech
Pathology Core Facility
Robert. H. Lurie Cancer Center
Northwestern University
710 N Fairbanks Court
Olson 8-421
Chicago,IL 60611
312-503-3723
b-frederick <@t> northwestern.edu<mailto:b-frederick <@t> northwestern.edu>
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------------------------------
Message: 10
Date: Tue, 10 Mar 2015 12:12:05 -0400
From: Erin Sarricks <esarricks <@t> gmail.com>
Subject: [Histonet] IHC / Morphometry Technician wanted in Shenandoah
Valley Virginia
To: histonet <histonet <@t> lists.utsouthwestern.edu>
Message-ID:
<CAPapK_1E+KJTXn9pLbWgLsVrd98Fdi1=ULpmhsbkSSdb8nrvQA <@t> mail.gmail.com>
Content-Type: text/plain; charset=UTF-8
Histology Laboratory located in the Shenandoah Valley of Virginia is
looking to add to it's team. In this position, you will need a working
knowledge of IHC theory and practical IHC experience. The best candidate
for the position will oversee immunohistochemical staining as well as
perform other histology functions including trimming of specimens, paraffin
embedding, microtomy and microscopic QC of slides. Experience with
morphometry is preferable.
Desirable candidates will possess the following:
- HT (ASCP) or QIHC registration preferred
- 4 years of Histology experience
- 1+ years of immunohistochemistry and/or immunofluorescence experience
- Keeps abreast with company's current policies and immunohistochemistry
technical updates and procedures
- Must be able to work independently and in a team environment
Full time employment benefits include subsidized medical and dental
insurance, vacation, holiday pay, and 401k after 1 year of employment.
Compensation is commensurate with experience.
If you are interested in this position, please respond to this post with
your resume and cover letter.
------------------------------
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End of Histonet Digest, Vol 136, Issue 12
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