[Histonet] Animal reference material

Wed Apr 1 13:37:04 CDT 2015

Totally agree with Andi, Staining is not much different, but processing
times are much shorter and very varied depending on size and type of
tissues. Embryo's being the shortest, and brain (typically) being the
longest.

The animal processing protocol from NSH was very very useful to me:
it is on this page:
http://www.nsh.org/content/textbooks-and-manuals

NSH VIR Manual
By the NSH VIR Committee
This manual, developed by the NSH VIR Committee, provides self-help
guidelines for animal tissue processing. The manual is a collection of
processing protocols and comments about animal tissue processing that have
been used in veterinary diagnostic, research, toxicology and marine
biology.  This book is available through the NSH Marketplace
<http://www.nsh.org/mynshtemp>. A discount is available to NSH members.

For cutting the tissues can be a bit dry, especially mouse liver, you just
need to soak it a bit more than you would human tissue,

good luck!

yours,
Caroline

On Tue, Mar 31, 2015 at 10:03 AM, Grantham, Andrea L - (algranth) <
algranth <@t> email.arizona.edu> wrote:

> After working for over twenty years on human tissues in clinical labs I
> went to work in a research core lab. I was the only histotech so I had to
> learn about animal, insect and plant tissue plus some things like cell
> pellets and films. I thank my connections from networking thru NSH and
> state societies who always came through with the perfect advice so don't
> NOT use this resource.
> I found that there wasn't much difference with routine special stains like
> trichrome, PAS, LFB, oil red o, etc. You may be asked to do some that
> aren't usually done on human tissue. I did a lot of picrosirius red and a
> special elastic stain for lung tissue. I was asked to combine some stains
> like H&E-alcian blue. It really depends on what the people do who are
> bringing you the tissue. Since my lab served researchers I could expect
> almost anything! Freida Carson's book is a good book to have on hand. I
> liked the second edition most. If you can get your hands on an old AFIP
> manual and a Preece get them!
> You may find differences with IHC. Can't help you there since that was
> done in another lab.
> Processing is where the differences lie. Make sure you get some good info
> on processing other species. Humason is good and don't forget the NSH has
> an Animal Processing Manual. I found a lot of protocols online. I had
> different schedules for different species and types of tissue - like mouse
> embryo or chick embryo, brains, bones, tiny little things like cultured
> mouse ovaries and on and on.
> Good luck!
>
> Andi G.
> Happily retired but soon to return to Histo (just a little)
> ________________________________________
> From: histonet-bounces <@t> lists.utsouthwestern.edu [
> histonet-bounces <@t> lists.utsouthwestern.edu] on behalf of Wineman, Terra [
> Terra.Wineman <@t> novusint.com]
> Sent: Tuesday, March 31, 2015 6:52 AM
> To: Patrick Laurie; Kimberly Marshall
> Cc: histonet <@t> lists.utsouthwestern.edu
> Subject: RE: [Histonet] Animal reference material
>
> Here is a link I found on google.
> https://archive.org/details/animaltissuetech00huma
>
> Terra Wineman, HTL (ASCP)CM
> Research Biologist, Physiology
> 636-926-7476 phone
> terra.wineman <@t> novusint.com
>
>
> -----Original Message-----
> From: histonet-bounces <@t> lists.utsouthwestern.edu [mailto:
> histonet-bounces <@t> lists.utsouthwestern.edu] On Behalf Of Patrick Laurie
> Sent: Tuesday, March 31, 2015 8:18 AM
> To: Kimberly Marshall
> Cc: histonet <@t> lists.utsouthwestern.edu
> Subject: Re: [Histonet] Animal reference material
>
> The best that I have used in the past was humason's animal tissue
> techniques.  I think that there are several editions out there, I used a
> 1979 edition.  After googling it, I see that there may be a full text
> version available online too.
>
> Patrick Laurie(HT)ASCP QIHC
>
> Histology Manager
>
> Celligent Diagnostics, LLC
>
> 101 East W.T. Harris Blvd  | Suite 1212 | Charlotte, NC 28262
>
> Work: 704-970-3300  Cell: 704-266-0869
>
> On Mon, Mar 30, 2015 at 12:49 PM, Kimberly Marshall <
> Kimberly <@t> animalreferencepathology.com> wrote:
>
> > ?Happy Monday all...
> >
> > Writing to ask if any of my fellow histo folks can suggest any
> > material/books or any information on staining animal tissue.  I am
> > bringing many special stains into the lab and being still new to
> > animal pathology I would like to see if there is any information out
> > there so I can educate myself.
> >
> > Thanks in advcance
> >
> > Kimberly
> >
> >
> >
> >
> > Kimberly Marshall H.T.(ASCP)
> >
> > Histology/Lab Supervisor
> >
> > Toll Free 1-800-426-2099
> >
> > Fax 801-584-5104
> >
> > PO Box 17580
> >
> > Salt Lake City, Utah 84107
> >
> > www.animalreferencepathology.com<http://www.animalreferencepathology.c
> > om/>
> >
> >
> >
> > Advancing the art and science of veterinary medicine
> >
> >
> >
> > [cid:image001.jpg <@t> 01CF8F87.A0BD4830]
> >
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-- 
Caroline Miller
Director of Histology
3Scan.com
415 2187297