[Histonet] RE: Negative Control for Validation

[Jamal]

Hi Linda
can you give us more details about the controls selection and processing
procedures.



Best Regards,


Jamal M. Al Rowaihi	Anatomic Pathology Supervisor       | Al Borg
Medical Laboratories |  Mobile +966 503629832|
j.rowaihi <@t> alborglaboratories.com 
Palestine St, Al Rajhi Building, P.O. Box 52817, Jeddah 21573, KSA    |
Phone: +966 12 670 0099	      | Fax: +966 12 676 4984     |
www.alborglaboratories.com

-----Original Message-----
From: histonet-bounces <@t> lists.utsouthwestern.edu
[mailto:histonet-bounces <@t> lists.utsouthwestern.edu] On Behalf Of Sebree Linda
A
Sent: Tuesday, September 30, 2014 12:43 AM
To: 'Arrington, Karla A'; 'histonet <@t> lists.utsouthwestern.edu'
Subject: [Histonet] RE: Negative Control for Validation

Hey Karla,

We use Vimentin as a positive reagent control, our negative control serum
for the negative reagent control and for the negative tissue control, the
pathologist reviews the antibody stained slide and OK's the internal
negative tissue elements as appropriately negative.

Hope that helps,

Linda A. Sebree 
University of Wisconsin Hospital & Clinics 
IHC/ISH Laboratory 
600 Highland Ave. 
Madison, WI 53792 
(608)265-6596 
FAX: (608)262-7174 

-----Original Message-----
From: histonet-bounces <@t> lists.utsouthwestern.edu
[mailto:histonet-bounces <@t> lists.utsouthwestern.edu] On Behalf Of Arrington,
Karla A
Sent: Monday, September 29, 2014 1:25 PM
To: 'histonet <@t> lists.utsouthwestern.edu'
Subject: [Histonet] Negative Control for Validation

We are beginning the process of validating new antibodies using the
BenchMark XT but have some questions for validation.

Question:  For a positive reagent control that stains appropriately
positive, is a known control block used? If not, what is used?
Question:  For a negative reagent control that stains appropriately
negative, what is ran?
Question:  Is a negative tissue slide ran for all of our 10 cases per
antibody, or per case that will be used for validation?

Thanks All!
Karla

Karla Arrington, HT(ASCP), HIT(AHIMA)
Lead Histology Technician
ANMC Pathology
907-729-1810
kaarrington <@t> anthc.org

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