[Histonet] RE: Negative Control for Validation

[Sebree Linda A]

Jamal,

We use the same patient cases throughout the validation procedure.  So each block gets stained with the new antibody (optimized protocol already signed off by IHC director or designee), vimentin (+ reagent control) and negative control serum (- reagent control).  As stated earlier, the negative tissue control is any non-staining negative tissue elements, expected to be negative, within the block.  

If you're interested I can send you our Validation Protocol Proposal form under separate cover.

Linda

Linda A. Sebree 
University of Wisconsin Hospital & Clinics 
IHC/ISH Laboratory 
600 Highland Ave. 
Madison, WI 53792 
(608)265-6596 
FAX: (608)262-7174 


-----Original Message-----
From: Jamal [mailto:j.rowaihi <@t> alborglaboratories.com] 
Sent: Tuesday, September 30, 2014 5:15 AM
To: Sebree Linda A; 'Arrington, Karla A'; histonet <@t> lists.utsouthwestern.edu
Subject: RE: [Histonet] RE: Negative Control for Validation

Hi Linda
can you give us more details about the controls selection and processing procedures.



Best Regards,


Jamal M. Al Rowaihi	Anatomic Pathology Supervisor       | Al Borg
Medical Laboratories |  Mobile +966 503629832| j.rowaihi <@t> alborglaboratories.com 
Palestine St, Al Rajhi Building, P.O. Box 52817, Jeddah 21573, KSA    |
Phone: +966 12 670 0099	      | Fax: +966 12 676 4984     |
www.alborglaboratories.com

-----Original Message-----
From: histonet-bounces <@t> lists.utsouthwestern.edu
[mailto:histonet-bounces <@t> lists.utsouthwestern.edu] On Behalf Of Sebree Linda A
Sent: Tuesday, September 30, 2014 12:43 AM
To: 'Arrington, Karla A'; 'histonet <@t> lists.utsouthwestern.edu'
Subject: [Histonet] RE: Negative Control for Validation

Hey Karla,

We use Vimentin as a positive reagent control, our negative control serum for the negative reagent control and for the negative tissue control, the pathologist reviews the antibody stained slide and OK's the internal negative tissue elements as appropriately negative.

Hope that helps,

Linda A. Sebree
University of Wisconsin Hospital & Clinics IHC/ISH Laboratory
600 Highland Ave. 
Madison, WI 53792
(608)265-6596
FAX: (608)262-7174 

-----Original Message-----
From: histonet-bounces <@t> lists.utsouthwestern.edu
[mailto:histonet-bounces <@t> lists.utsouthwestern.edu] On Behalf Of Arrington, Karla A
Sent: Monday, September 29, 2014 1:25 PM
To: 'histonet <@t> lists.utsouthwestern.edu'
Subject: [Histonet] Negative Control for Validation

We are beginning the process of validating new antibodies using the BenchMark XT but have some questions for validation.

Question:  For a positive reagent control that stains appropriately positive, is a known control block used? If not, what is used?
Question:  For a negative reagent control that stains appropriately negative, what is ran?
Question:  Is a negative tissue slide ran for all of our 10 cases per antibody, or per case that will be used for validation?

Thanks All!
Karla

Karla Arrington, HT(ASCP), HIT(AHIMA)
Lead Histology Technician
ANMC Pathology
907-729-1810
kaarrington <@t> anthc.org

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