[Histonet] RE: Three shades of Eosin

[Michael Ann Jones]

Terri - agreed. We do the same.
Michael Ann Jones, HT (ASCP)
Histology Manager
Metropath
7444 W. Alaska Dr. #250
Lakewood, CO 80226
303.634.2511
Mjones <@t> metropath.com




On 9/11/14, 12:42 PM, "Terri  Braud" <tbraud <@t> holyredeemer.com> wrote:

>Eosin is differentiated by water content immediately following the Eosin
>stain.  This can be accomplished by a water rinse, or rinse in a diluted
>alcohol.  I prefer using 70% Alcohol to 95% Alcohol, then 3 washes of
>Absolute, all timed to give the desired differentiation for the 3
>desired shades.
>
>Terri L. Braud, HT(ASCP)
>Anatomic Pathology Supervisor
>Holy Redeemer Hospital Laboratory
>1648 Huntingdon Pike
>Meadowbrook, PA 19046
>Ph: 215-938-3676
>Fax: 215-938-3874
>
>Sent: Thursday, September 11, 2014 12:09 PM
>To: histonet <@t> lists.utsouthwestern.edu
>Subject: Histonet Digest, Vol 130, Issue 11
>
>   8. Eosin shades (Manahil)
>Date: Thu, 11 Sep 2014 20:15:21 +0400
>From: Manahil <mbireir <@t> yahoo.com>
>Subject: [Histonet] Eosin shades
>Hi every one
>Any one know how to get 3 shades of eosin stain. I am using alcoholic
>eosin.
>Thanks
>Manahil Elbireir
>HTL/ ASCP
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