Re: [Histonet] RE: Three shades of Eosin

[wsimons <@t> athensgastro.com]

Ok, I can't help myself....but all this talk is reminding me of " 50 shades of grey"....
(can't put this on Histonet)

I'll take my "HistoPorn" any day of the week over 50 shades!  
All the pretty pictures under the scope. That is my passion~
I get my "3 shades of eosin" with Richard Allan eosin Y.

Wanda K Simons, HT(ASCP)



>  -------Original Message-------
>  From: Michael Ann Jones <mjones <@t> metropath.com>
>  To: Terri Braud <tbraud <@t> holyredeemer.com>, histonet <@t> lists.utsouthwestern.edu <histonet <@t> lists.utsouthwestern.edu>
>  Subject: Re: [Histonet] RE: Three shades of Eosin
>  Sent: Sep 11 '14 15:03
>  
>  Terri - agreed. We do the same.
>  Michael Ann Jones, HT (ASCP)
>  Histology Manager
>  Metropath
>  7444 W. Alaska Dr. #250
>  Lakewood, CO 80226
>  303.634.2511
>  Mjones <@t> metropath.com
>  
>  
>  
>  
>  On 9/11/14, 12:42 PM, "Terri  Braud" <tbraud <@t> holyredeemer.com> wrote:
>  
>  >Eosin is differentiated by water content immediately following the Eosin
>  >stain.  This can be accomplished by a water rinse, or rinse in a diluted
>  >alcohol.  I prefer using 70% Alcohol to 95% Alcohol, then 3 washes of
>  >Absolute, all timed to give the desired differentiation for the 3
>  >desired shades.
>  >
>  >Terri L. Braud, HT(ASCP)
>  >Anatomic Pathology Supervisor
>  >Holy Redeemer Hospital Laboratory
>  >1648 Huntingdon Pike
>  >Meadowbrook, PA 19046
>  >Ph: 215-938-3676
>  >Fax: 215-938-3874
>  >
>  >Sent: Thursday, September 11, 2014 12:09 PM
>  >To: histonet <@t> lists.utsouthwestern.edu
>  >Subject: Histonet Digest, Vol 130, Issue 11
>  >
>  >   8. Eosin shades (Manahil)
>  >Date: Thu, 11 Sep 2014 20:15:21 +0400
>  >From: Manahil <mbireir <@t> yahoo.com>
>  >Subject: [Histonet] Eosin shades
>  >Hi every one
>  >Any one know how to get 3 shades of eosin stain. I am using alcoholic
>  >eosin.
>  >Thanks
>  >Manahil Elbireir
>  >HTL/ ASCP
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