[Histonet] RE: Three shades of Eosin

[Terri Braud]

Eosin is differentiated by water content immediately following the Eosin
stain.  This can be accomplished by a water rinse, or rinse in a diluted
alcohol.  I prefer using 70% Alcohol to 95% Alcohol, then 3 washes of
Absolute, all timed to give the desired differentiation for the 3
desired shades.

Terri L. Braud, HT(ASCP)
Anatomic Pathology Supervisor
Holy Redeemer Hospital Laboratory
1648 Huntingdon Pike
Meadowbrook, PA 19046
Ph: 215-938-3676
Fax: 215-938-3874

Sent: Thursday, September 11, 2014 12:09 PM
To: histonet <@t> lists.utsouthwestern.edu
Subject: Histonet Digest, Vol 130, Issue 11

   8. Eosin shades (Manahil)
Date: Thu, 11 Sep 2014 20:15:21 +0400
From: Manahil <mbireir <@t> yahoo.com>
Subject: [Histonet] Eosin shades
Hi every one
Any one know how to get 3 shades of eosin stain. I am using alcoholic
eosin.
Thanks
Manahil Elbireir
HTL/ ASCP
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