[Histonet] Antibody deposit visualization

[Julio Benavides]

Hi,

may be you can try to label murine immunoglobulins. A mere secondary  
against murine IgGs could do the trick, depending on which isotype  
your client is suspecting to form the precipitates.

Just an idea, never have tried before.

Cheers

Julio



"Rodriguez, Kristofer" <Kristofer.Rodriguez <@t> bcm.edu> escribió:

> One of my clients has been performing experiments with a mouse model  
> of Dengue Virus Infection. They have been treating the mice with  
> monoclonal antibodies (client did not specify what antibody)as a  
> potential treatment for dengue virus infection. They have harvested  
> the kidneys and put them in 10% NBF. They want to know if routine  
> H&E will show any antibody deposits.
>
> I told him that I would not be able to directly observe the antibody  
> deposit itself, however I might be able to observe the reaction to  
> the antibody deposit in the basement membrane of the glomeruli as a  
> membranous glomerulopathy on an H&E.
>
> I was wondering if I should do any special stains that might show  
> more than an H&E.
> _______________________________________________
> Histonet mailing list
> Histonet <@t> lists.utsouthwestern.edu
> http://lists.utsouthwestern.edu/mailman/listinfo/histonet