[Histonet] Antibody deposit visualization

[Rene J Buesa]

Depending on the reaction of the glomerulii to the Dengue virus and the stage of such reaction perhaps a PAM (periodic acid methenamine silver) stain could show something.René J.  

     On Wednesday, October 22, 2014 11:31 AM, "Rodriguez, Kristofer" <Kristofer.Rodriguez <@t> bcm.edu> wrote:
   

 One of my clients has been performing experiments with a mouse model of Dengue Virus Infection. They have been treating the mice with monoclonal antibodies (client did not specify what antibody)as a potential treatment for dengue virus infection. They have harvested the kidneys and put them in 10% NBF. They want to know if routine H&E will show any antibody deposits.

I told him that I would not be able to directly observe the antibody deposit itself, however I might be able to observe the reaction to the antibody deposit in the basement membrane of the glomeruli as a membranous glomerulopathy on an H&E.

I was wondering if I should do any special stains that might show more than an H&E.
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