[Histonet] RE: Antibody deposit visualization

[Elizabeth Chlipala]

This was years ago be we stained for the virus via IHC.  The antibody was supplied by the client.  That would seem to me to be the best method to determine deposits.

Liz

Elizabeth A. Chlipala, BS, HTL(ASCP)QIHC
Premier Laboratory, LLC
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liz <@t> premierlab.com
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-----Original Message-----
From: histonet-bounces <@t> lists.utsouthwestern.edu [mailto:histonet-bounces <@t> lists.utsouthwestern.edu] On Behalf Of Rodriguez, Kristofer
Sent: Wednesday, October 22, 2014 9:31 AM
To: histonet <@t> lists.utsouthwestern.edu
Subject: [Histonet] Antibody deposit visualization

One of my clients has been performing experiments with a mouse model of Dengue Virus Infection. They have been treating the mice with monoclonal antibodies (client did not specify what antibody)as a potential treatment for dengue virus infection. They have harvested the kidneys and put them in 10% NBF. They want to know if routine H&E will show any antibody deposits.

I told him that I would not be able to directly observe the antibody deposit itself, however I might be able to observe the reaction to the antibody deposit in the basement membrane of the glomeruli as a membranous glomerulopathy on an H&E.

I was wondering if I should do any special stains that might show more than an H&E.
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